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RCAS-RNAi: A loss-of-function method for the developing chick retina
BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick ey...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402266/ https://www.ncbi.nlm.nih.gov/pubmed/16426460 http://dx.doi.org/10.1186/1471-213X-6-2 |
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author | Harpavat, Sanjiv Cepko, Constance L |
author_facet | Harpavat, Sanjiv Cepko, Constance L |
author_sort | Harpavat, Sanjiv |
collection | PubMed |
description | BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. CONCLUSION: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina. |
format | Text |
id | pubmed-1402266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-14022662006-03-16 RCAS-RNAi: A loss-of-function method for the developing chick retina Harpavat, Sanjiv Cepko, Constance L BMC Dev Biol Methodology Article BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. CONCLUSION: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina. BioMed Central 2006-01-22 /pmc/articles/PMC1402266/ /pubmed/16426460 http://dx.doi.org/10.1186/1471-213X-6-2 Text en Copyright © 2006 Harpavat and Cepko; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Harpavat, Sanjiv Cepko, Constance L RCAS-RNAi: A loss-of-function method for the developing chick retina |
title | RCAS-RNAi: A loss-of-function method for the developing chick retina |
title_full | RCAS-RNAi: A loss-of-function method for the developing chick retina |
title_fullStr | RCAS-RNAi: A loss-of-function method for the developing chick retina |
title_full_unstemmed | RCAS-RNAi: A loss-of-function method for the developing chick retina |
title_short | RCAS-RNAi: A loss-of-function method for the developing chick retina |
title_sort | rcas-rnai: a loss-of-function method for the developing chick retina |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402266/ https://www.ncbi.nlm.nih.gov/pubmed/16426460 http://dx.doi.org/10.1186/1471-213X-6-2 |
work_keys_str_mv | AT harpavatsanjiv rcasrnaialossoffunctionmethodforthedevelopingchickretina AT cepkoconstancel rcasrnaialossoffunctionmethodforthedevelopingchickretina |