Cargando…

RCAS-RNAi: A loss-of-function method for the developing chick retina

BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick ey...

Descripción completa

Detalles Bibliográficos
Autores principales: Harpavat, Sanjiv, Cepko, Constance L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402266/
https://www.ncbi.nlm.nih.gov/pubmed/16426460
http://dx.doi.org/10.1186/1471-213X-6-2
_version_ 1782127004990046208
author Harpavat, Sanjiv
Cepko, Constance L
author_facet Harpavat, Sanjiv
Cepko, Constance L
author_sort Harpavat, Sanjiv
collection PubMed
description BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. CONCLUSION: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.
format Text
id pubmed-1402266
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-14022662006-03-16 RCAS-RNAi: A loss-of-function method for the developing chick retina Harpavat, Sanjiv Cepko, Constance L BMC Dev Biol Methodology Article BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. CONCLUSION: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina. BioMed Central 2006-01-22 /pmc/articles/PMC1402266/ /pubmed/16426460 http://dx.doi.org/10.1186/1471-213X-6-2 Text en Copyright © 2006 Harpavat and Cepko; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Harpavat, Sanjiv
Cepko, Constance L
RCAS-RNAi: A loss-of-function method for the developing chick retina
title RCAS-RNAi: A loss-of-function method for the developing chick retina
title_full RCAS-RNAi: A loss-of-function method for the developing chick retina
title_fullStr RCAS-RNAi: A loss-of-function method for the developing chick retina
title_full_unstemmed RCAS-RNAi: A loss-of-function method for the developing chick retina
title_short RCAS-RNAi: A loss-of-function method for the developing chick retina
title_sort rcas-rnai: a loss-of-function method for the developing chick retina
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402266/
https://www.ncbi.nlm.nih.gov/pubmed/16426460
http://dx.doi.org/10.1186/1471-213X-6-2
work_keys_str_mv AT harpavatsanjiv rcasrnaialossoffunctionmethodforthedevelopingchickretina
AT cepkoconstancel rcasrnaialossoffunctionmethodforthedevelopingchickretina