Cargando…
Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation
INTRODUCTION: The aim of this study was to collect serial samples of nipple aspirate (NA) and ductal lavage (DL) fluid from women with germline BRCA1/2 mutations in order to create a biorepository for use in identifying biomarkers of breast cancer risk. METHODS: Between March 2003 and February 2005,...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1410769/ https://www.ncbi.nlm.nih.gov/pubmed/16457692 http://dx.doi.org/10.1186/bcr1348 |
_version_ | 1782127070240833536 |
---|---|
author | Mitchell, Gillian Antill, Yoland C Murray, William Kirk, Judy Salisbury, Elizabeth Lindeman, Geoffrey J Di Iulio, Juliana Milner, Alvin D Devereaux, Lisa Phillips, Kelly-Anne |
author_facet | Mitchell, Gillian Antill, Yoland C Murray, William Kirk, Judy Salisbury, Elizabeth Lindeman, Geoffrey J Di Iulio, Juliana Milner, Alvin D Devereaux, Lisa Phillips, Kelly-Anne |
author_sort | Mitchell, Gillian |
collection | PubMed |
description | INTRODUCTION: The aim of this study was to collect serial samples of nipple aspirate (NA) and ductal lavage (DL) fluid from women with germline BRCA1/2 mutations in order to create a biorepository for use in identifying biomarkers of breast cancer risk. METHODS: Between March 2003 and February 2005, 52 women with germline BRCA1 or BRCA2 mutations (median age 43 years, range 27 to 65 years) were scheduled for six-monthly NA, DL and venesection. DL was attempted for all NA fluid-yielding (FY) and any non-FY ducts that could be located at each visit. RESULTS: Twenty-seven (52%) women were postmenopausal, predominantly (19/27) from risk reducing bilateral salpingo-oophorectomy (BSO). FY ducts were identified in 60% of all women, 76% of premenopausal women versus 44% of postmenopausal (P = 0.026). Eighty-five percent of women had successful DL. Success was most likely in women with FY ducts (FY 94% versus non-FY 71% (P = 0.049). DL samples were more likely to be cellular if collected from FY ducts (FY 68% versus non-FY 43%; P = 0.037). Total cell counts were associated with FY status (FY median cell count 30,996, range 0 to >1,000,000 versus non-FY median cell count 0, range 0 to 173,577; P = 0.002). Four women (8%) had ducts with severe atypia with or without additional ducts with mild epithelial atypia; seven others had ducts with mild atypia alone (11/52 (21%) in total). Median total cell count was greater from ducts with atypia (105,870, range 1920 to >1,000,000) than those with no atypia (174, 0 to >1,000,000; P ≤ 0.001). CONCLUSION: It is feasible to collect serial NA and DL samples from women at high genetic risk of breast cancer, and we are creating a unique, prospective collection of ductal samples that have the potential to be used for discovery of biomarkers of breast cancer risk and evaluate the ongoing effects of risk reducing BSO. DL cellular atypia was not predictive of a current breast cancer and longer follow up is needed to determine whether atypia is an additional marker of future breast cancer risk in this population already at high genetic risk of breast cancer. |
format | Text |
id | pubmed-1410769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-14107692006-03-24 Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation Mitchell, Gillian Antill, Yoland C Murray, William Kirk, Judy Salisbury, Elizabeth Lindeman, Geoffrey J Di Iulio, Juliana Milner, Alvin D Devereaux, Lisa Phillips, Kelly-Anne Breast Cancer Res Research Article INTRODUCTION: The aim of this study was to collect serial samples of nipple aspirate (NA) and ductal lavage (DL) fluid from women with germline BRCA1/2 mutations in order to create a biorepository for use in identifying biomarkers of breast cancer risk. METHODS: Between March 2003 and February 2005, 52 women with germline BRCA1 or BRCA2 mutations (median age 43 years, range 27 to 65 years) were scheduled for six-monthly NA, DL and venesection. DL was attempted for all NA fluid-yielding (FY) and any non-FY ducts that could be located at each visit. RESULTS: Twenty-seven (52%) women were postmenopausal, predominantly (19/27) from risk reducing bilateral salpingo-oophorectomy (BSO). FY ducts were identified in 60% of all women, 76% of premenopausal women versus 44% of postmenopausal (P = 0.026). Eighty-five percent of women had successful DL. Success was most likely in women with FY ducts (FY 94% versus non-FY 71% (P = 0.049). DL samples were more likely to be cellular if collected from FY ducts (FY 68% versus non-FY 43%; P = 0.037). Total cell counts were associated with FY status (FY median cell count 30,996, range 0 to >1,000,000 versus non-FY median cell count 0, range 0 to 173,577; P = 0.002). Four women (8%) had ducts with severe atypia with or without additional ducts with mild epithelial atypia; seven others had ducts with mild atypia alone (11/52 (21%) in total). Median total cell count was greater from ducts with atypia (105,870, range 1920 to >1,000,000) than those with no atypia (174, 0 to >1,000,000; P ≤ 0.001). CONCLUSION: It is feasible to collect serial NA and DL samples from women at high genetic risk of breast cancer, and we are creating a unique, prospective collection of ductal samples that have the potential to be used for discovery of biomarkers of breast cancer risk and evaluate the ongoing effects of risk reducing BSO. DL cellular atypia was not predictive of a current breast cancer and longer follow up is needed to determine whether atypia is an additional marker of future breast cancer risk in this population already at high genetic risk of breast cancer. BioMed Central 2005 2005-11-14 /pmc/articles/PMC1410769/ /pubmed/16457692 http://dx.doi.org/10.1186/bcr1348 Text en Copyright © 2005 Mitchell et al.; licensee BioMed Central Ltd. |
spellingShingle | Research Article Mitchell, Gillian Antill, Yoland C Murray, William Kirk, Judy Salisbury, Elizabeth Lindeman, Geoffrey J Di Iulio, Juliana Milner, Alvin D Devereaux, Lisa Phillips, Kelly-Anne Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title | Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title_full | Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title_fullStr | Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title_full_unstemmed | Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title_short | Nipple aspiration and ductal lavage in women with a germline BRCA1 or BRCA2 mutation |
title_sort | nipple aspiration and ductal lavage in women with a germline brca1 or brca2 mutation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1410769/ https://www.ncbi.nlm.nih.gov/pubmed/16457692 http://dx.doi.org/10.1186/bcr1348 |
work_keys_str_mv | AT mitchellgillian nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT antillyolandc nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT murraywilliam nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT kirkjudy nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT salisburyelizabeth nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT lindemangeoffreyj nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT diiuliojuliana nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT milneralvind nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT devereauxlisa nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation AT phillipskellyanne nippleaspirationandductallavageinwomenwithagermlinebrca1orbrca2mutation |