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Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock
The transcriptional circuits of circadian clocks control physiological and behavioral rhythms. Light may affect such overt rhythms in two ways: (1) by entraining the clock circuits and (2) via clock-independent molecular pathways. In this study we examine the relationship between autonomous transcri...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413497/ https://www.ncbi.nlm.nih.gov/pubmed/16565745 http://dx.doi.org/10.1371/journal.pgen.0020039 |
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author | Wijnen, Herman Naef, Felix Boothroyd, Catharine Claridge-Chang, Adam Young, Michael W |
author_facet | Wijnen, Herman Naef, Felix Boothroyd, Catharine Claridge-Chang, Adam Young, Michael W |
author_sort | Wijnen, Herman |
collection | PubMed |
description | The transcriptional circuits of circadian clocks control physiological and behavioral rhythms. Light may affect such overt rhythms in two ways: (1) by entraining the clock circuits and (2) via clock-independent molecular pathways. In this study we examine the relationship between autonomous transcript oscillations and light-driven transcript responses. Transcript profiles of wild-type and arrhythmic mutant Drosophila were recorded both in the presence of an environmental photocycle and in constant darkness. Systematic autonomous oscillations in the 12- to 48-h period range were detectable only in wild-type flies and occurred preferentially at the circadian period length. However, an extensive program of light-driven expression was confirmed in arrhythmic mutant flies. Many light-responsive transcripts are preferentially expressed in the compound eyes and the phospholipase C component of phototransduction, NORPA (no receptor potential), is required for their light-dependent regulation. Although there is evidence for the existence of multiple molecular clock circuits in cyanobacteria, protists, plants, and fungi, Drosophila appears to possess only one such system. The sustained photic expression responses identified here are partially coupled to the circadian clock and may reflect a mechanism for flies to modulate functions such as visual sensitivity and synaptic transmission in response to seasonal changes in photoperiod. |
format | Text |
id | pubmed-1413497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-14134972006-03-24 Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock Wijnen, Herman Naef, Felix Boothroyd, Catharine Claridge-Chang, Adam Young, Michael W PLoS Genet Research Article The transcriptional circuits of circadian clocks control physiological and behavioral rhythms. Light may affect such overt rhythms in two ways: (1) by entraining the clock circuits and (2) via clock-independent molecular pathways. In this study we examine the relationship between autonomous transcript oscillations and light-driven transcript responses. Transcript profiles of wild-type and arrhythmic mutant Drosophila were recorded both in the presence of an environmental photocycle and in constant darkness. Systematic autonomous oscillations in the 12- to 48-h period range were detectable only in wild-type flies and occurred preferentially at the circadian period length. However, an extensive program of light-driven expression was confirmed in arrhythmic mutant flies. Many light-responsive transcripts are preferentially expressed in the compound eyes and the phospholipase C component of phototransduction, NORPA (no receptor potential), is required for their light-dependent regulation. Although there is evidence for the existence of multiple molecular clock circuits in cyanobacteria, protists, plants, and fungi, Drosophila appears to possess only one such system. The sustained photic expression responses identified here are partially coupled to the circadian clock and may reflect a mechanism for flies to modulate functions such as visual sensitivity and synaptic transmission in response to seasonal changes in photoperiod. Public Library of Science 2006-03 2006-03-24 /pmc/articles/PMC1413497/ /pubmed/16565745 http://dx.doi.org/10.1371/journal.pgen.0020039 Text en © 2006 Wijnen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wijnen, Herman Naef, Felix Boothroyd, Catharine Claridge-Chang, Adam Young, Michael W Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title | Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title_full | Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title_fullStr | Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title_full_unstemmed | Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title_short | Control of Daily Transcript Oscillations in Drosophila by Light and the Circadian Clock |
title_sort | control of daily transcript oscillations in drosophila by light and the circadian clock |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413497/ https://www.ncbi.nlm.nih.gov/pubmed/16565745 http://dx.doi.org/10.1371/journal.pgen.0020039 |
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