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Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice

BACKGROUND: Lim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at...

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Autores principales: Chen, You-Tzung, Kobayashi, Akio, Kwan, Kin Ming, Johnson, Randy L, Behringer, Richard R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413522/
https://www.ncbi.nlm.nih.gov/pubmed/16464245
http://dx.doi.org/10.1186/1471-2369-7-1
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author Chen, You-Tzung
Kobayashi, Akio
Kwan, Kin Ming
Johnson, Randy L
Behringer, Richard R
author_facet Chen, You-Tzung
Kobayashi, Akio
Kwan, Kin Ming
Johnson, Randy L
Behringer, Richard R
author_sort Chen, You-Tzung
collection PubMed
description BACKGROUND: Lim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at the nephric vesicle stage, leading to the production of small, non-functional kidneys that lack nephrons. METHODS: In the present study, we used Affymetrix probe arrays to screen for nephron-specific genes by comparing the expression profiles of control and Lim1 conditional mutant kidneys. Kidneys from two developmental stages, embryonic day 14.5 (E14.5) and 18.5 (E18.5), were examined. RESULTS: Comparison of E18.5 kidney expression profiles generated a list of 465 nephron-specific gene candidates that showed a more than 2-fold increase in their expression level in control kidney versus the Lim1 conditional mutant kidney. Computational analysis confirmed that this screen enriched for kidney-specific genes. Furthermore, at least twenty-eight of the top fifty (56%) candidates (or their vertebrate orthologs) were previously reported to have a nephron-specific expression pattern. Our analysis of E14.5 expression data yielded 41 candidate genes that are up-regulated in the control kidneys compared to the conditional mutants. Three of them are related to the Notch signaling pathway that is known to be important in cell fate determination and nephron patterning. CONCLUSION: Therefore, we demonstrate that Lim1 conditional mutant kidneys serve as a novel tissue source for comprehensive expression studies and provide a means to identify nephron-specific genes.
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spelling pubmed-14135222006-03-25 Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice Chen, You-Tzung Kobayashi, Akio Kwan, Kin Ming Johnson, Randy L Behringer, Richard R BMC Nephrol Research Article BACKGROUND: Lim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at the nephric vesicle stage, leading to the production of small, non-functional kidneys that lack nephrons. METHODS: In the present study, we used Affymetrix probe arrays to screen for nephron-specific genes by comparing the expression profiles of control and Lim1 conditional mutant kidneys. Kidneys from two developmental stages, embryonic day 14.5 (E14.5) and 18.5 (E18.5), were examined. RESULTS: Comparison of E18.5 kidney expression profiles generated a list of 465 nephron-specific gene candidates that showed a more than 2-fold increase in their expression level in control kidney versus the Lim1 conditional mutant kidney. Computational analysis confirmed that this screen enriched for kidney-specific genes. Furthermore, at least twenty-eight of the top fifty (56%) candidates (or their vertebrate orthologs) were previously reported to have a nephron-specific expression pattern. Our analysis of E14.5 expression data yielded 41 candidate genes that are up-regulated in the control kidneys compared to the conditional mutants. Three of them are related to the Notch signaling pathway that is known to be important in cell fate determination and nephron patterning. CONCLUSION: Therefore, we demonstrate that Lim1 conditional mutant kidneys serve as a novel tissue source for comprehensive expression studies and provide a means to identify nephron-specific genes. BioMed Central 2006-02-07 /pmc/articles/PMC1413522/ /pubmed/16464245 http://dx.doi.org/10.1186/1471-2369-7-1 Text en Copyright © 2006 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, You-Tzung
Kobayashi, Akio
Kwan, Kin Ming
Johnson, Randy L
Behringer, Richard R
Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title_full Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title_fullStr Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title_full_unstemmed Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title_short Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice
title_sort gene expression profiles in developing nephrons using lim1 metanephric mesenchyme-specific conditional mutant mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413522/
https://www.ncbi.nlm.nih.gov/pubmed/16464245
http://dx.doi.org/10.1186/1471-2369-7-1
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