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Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites
BACKGROUND: Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In the Creator system, an ORF introduced into a donor vector can be transferred with...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421398/ https://www.ncbi.nlm.nih.gov/pubmed/16519801 http://dx.doi.org/10.1186/1472-6750-6-13 |