NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation

The transfer-messenger RNA (tmRNA) pseudoknot PK1 is essential for bacterial trans-translation, a ribosomal rescue mechanism. We report the solution structure of PK1 from Aquifex aeolicus, which despite an unprecedented small number of nucleotides and thus an unprecented compact size, displays a ver...

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Detalles Bibliográficos
Autores principales: Nonin-Lecomte, Sylvie, Felden, Brice, Dardel, Frédéric
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1428798/
https://www.ncbi.nlm.nih.gov/pubmed/16595798
http://dx.doi.org/10.1093/nar/gkl111
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author Nonin-Lecomte, Sylvie
Felden, Brice
Dardel, Frédéric
author_facet Nonin-Lecomte, Sylvie
Felden, Brice
Dardel, Frédéric
author_sort Nonin-Lecomte, Sylvie
collection PubMed
description The transfer-messenger RNA (tmRNA) pseudoknot PK1 is essential for bacterial trans-translation, a ribosomal rescue mechanism. We report the solution structure of PK1 from Aquifex aeolicus, which despite an unprecedented small number of nucleotides and thus an unprecented compact size, displays a very high thermal stability. Several unusual structural features account for these properties and indicate that PK1 belongs to the class of ribosomal frameshift pseudoknots. This suggests a similarity between the mechanism of programmed ribosomal frameshifting and trans-translation.
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spelling pubmed-14287982006-04-12 NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation Nonin-Lecomte, Sylvie Felden, Brice Dardel, Frédéric Nucleic Acids Res Article The transfer-messenger RNA (tmRNA) pseudoknot PK1 is essential for bacterial trans-translation, a ribosomal rescue mechanism. We report the solution structure of PK1 from Aquifex aeolicus, which despite an unprecedented small number of nucleotides and thus an unprecented compact size, displays a very high thermal stability. Several unusual structural features account for these properties and indicate that PK1 belongs to the class of ribosomal frameshift pseudoknots. This suggests a similarity between the mechanism of programmed ribosomal frameshifting and trans-translation. Oxford University Press 2006 2006-04-04 /pmc/articles/PMC1428798/ /pubmed/16595798 http://dx.doi.org/10.1093/nar/gkl111 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Nonin-Lecomte, Sylvie
Felden, Brice
Dardel, Frédéric
NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title_full NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title_fullStr NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title_full_unstemmed NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title_short NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation
title_sort nmr structure of the aquifex aeolicus tmrna pseudoknot pk1: new insights into the recoding event of the ribosomal trans-translation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1428798/
https://www.ncbi.nlm.nih.gov/pubmed/16595798
http://dx.doi.org/10.1093/nar/gkl111
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AT dardelfrederic nmrstructureoftheaquifexaeolicustmrnapseudoknotpk1newinsightsintotherecodingeventoftheribosomaltranstranslation

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