Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine

Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS stra...

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Detalles Bibliográficos
Autores principales: Margalith, Michal, Vilalta, Adrián
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1431525/
https://www.ncbi.nlm.nih.gov/pubmed/16480501
http://dx.doi.org/10.1186/1479-0556-4-2
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author Margalith, Michal
Vilalta, Adrián
author_facet Margalith, Michal
Vilalta, Adrián
author_sort Margalith, Michal
collection PubMed
description Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS strain) was evaluated in vivo for ability to elicit neutralizing titers. pDNA:DMRIE-DOPE formulated at two DNA:cationic lipid molar ratios was compared in mice to a Vaxfectin™-pDNA formulation. Mouse data indicate that Vaxfectin™ is more effective than DMRIE-DOPE in eliciting neutralizing titers. In addition, the ratio of pDNA to DMRIE-DOPE can also affect neutralizing titers. Our data show that sustained neutralizing titers (120 days) can be obtained after a single administration of DMRIE-DOPE-formulated pDNA in rabbits.
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spelling pubmed-14315252006-04-06 Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine Margalith, Michal Vilalta, Adrián Genet Vaccines Ther Research Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS strain) was evaluated in vivo for ability to elicit neutralizing titers. pDNA:DMRIE-DOPE formulated at two DNA:cationic lipid molar ratios was compared in mice to a Vaxfectin™-pDNA formulation. Mouse data indicate that Vaxfectin™ is more effective than DMRIE-DOPE in eliciting neutralizing titers. In addition, the ratio of pDNA to DMRIE-DOPE can also affect neutralizing titers. Our data show that sustained neutralizing titers (120 days) can be obtained after a single administration of DMRIE-DOPE-formulated pDNA in rabbits. BioMed Central 2006-02-15 /pmc/articles/PMC1431525/ /pubmed/16480501 http://dx.doi.org/10.1186/1479-0556-4-2 Text en Copyright © 2006 Margalith and Vilalta; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Margalith, Michal
Vilalta, Adrián
Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title_full Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title_fullStr Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title_full_unstemmed Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title_short Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine
title_sort sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pdna vaccine
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1431525/
https://www.ncbi.nlm.nih.gov/pubmed/16480501
http://dx.doi.org/10.1186/1479-0556-4-2
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AT vilaltaadrian sustainedprotectiverabiesneutralizingantibodytitersafteradministrationofcationiclipidformulatedpdnavaccine

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