Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting

In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that...

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Detalles Bibliográficos
Autores principales: D'Andrea, Gabriele, Lizzi, Anna R, Venditti, Sara, Di Francesco, Laura, Giorgi, Alessandra, Mignogna, Giuseppina, Oratore, Arduino, Bozzi, Argante
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435870/
https://www.ncbi.nlm.nih.gov/pubmed/16571109
http://dx.doi.org/10.1186/1477-5956-4-4
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author D'Andrea, Gabriele
Lizzi, Anna R
Venditti, Sara
Di Francesco, Laura
Giorgi, Alessandra
Mignogna, Giuseppina
Oratore, Arduino
Bozzi, Argante
author_facet D'Andrea, Gabriele
Lizzi, Anna R
Venditti, Sara
Di Francesco, Laura
Giorgi, Alessandra
Mignogna, Giuseppina
Oratore, Arduino
Bozzi, Argante
author_sort D'Andrea, Gabriele
collection PubMed
description In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.
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spelling pubmed-14358702006-04-14 Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting D'Andrea, Gabriele Lizzi, Anna R Venditti, Sara Di Francesco, Laura Giorgi, Alessandra Mignogna, Giuseppina Oratore, Arduino Bozzi, Argante Proteome Sci Research In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively. BioMed Central 2006-03-29 /pmc/articles/PMC1435870/ /pubmed/16571109 http://dx.doi.org/10.1186/1477-5956-4-4 Text en Copyright © 2006 D'Andrea et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
D'Andrea, Gabriele
Lizzi, Anna R
Venditti, Sara
Di Francesco, Laura
Giorgi, Alessandra
Mignogna, Giuseppina
Oratore, Arduino
Bozzi, Argante
Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title_full Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title_fullStr Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title_full_unstemmed Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title_short Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
title_sort proteins pattern alteration in azt-treated k562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435870/
https://www.ncbi.nlm.nih.gov/pubmed/16571109
http://dx.doi.org/10.1186/1477-5956-4-4
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