Interferon-γ increases neuronal death in response to amyloid-β(1-42)

BACKGROUND: Alzheimer's disease is a neurodegenerative disorder characterized by a progressive cognitive impairment, the consequence of neuronal dysfunction and ultimately the death of neurons. The amyloid hypothesis proposes that neuronal damage results from the accumulation of insoluble, hydrophobic, fibrillar peptides such as amyloid-β(1-42). These peptides activate enzymes resulting in a cascade of second messengers including prostaglandins and platelet-activating factor. Apoptosis of neurons is thought to follow as a consequence of the uncontrolled release of second messengers. Biochemical, histopathological and genetic studies suggest that pro-inflammatory cytokines play a role in neurodegeneration during Alzheimer's disease. In the current study we examined the effects of interferon (IFN)-γ, tumour necrosis factor (TNF)α, interleukin (IL)-1β and IL-6 on neurons. METHODS: Primary murine cortical or cerebellar neurons, or human SH-SY5Y neuroblastoma cells, were grown in vitro. Neurons were treated with cytokines prior to incubation with different neuronal insults. Cell survival, caspase-3 activity (a measure of apoptosis) and prostaglandin production were measured. Immunoblots were used to determine the effects of cytokines on the levels of cytoplasmic phospholipase A(2 )or phospholipase C γ-1. RESULTS: While none of the cytokines tested were directly neurotoxic, pre-treatment with IFN-γ sensitised neurons to the toxic effects of amyloid-β(1-42 )or HuPrP82-146 (a neurotoxic peptide found in prion diseases). The effects of IFN-γ were seen on cortical and cerebellar neurons, and on SH-SY5Y neuroblastoma cells. However, pre-treatment with IFN-γ did not affect the sensitivity to neurons treated with staurosporine or hydrogen peroxide. Pre-treatment with IFN-γ increased the levels of cytoplasmic phospholipase A(2 )in SH-SY5Y cells and increased prostaglandin E(2 )production in response to amyloid-β(1-42). CONCLUSION: Treatment of neuronal cells with IFN-γ increased neuronal death in response to amyloid-β(1-42 )or HuPrP82-146. IFN-γ increased the levels of cytoplasmic phospholipase A(2 )in cultured neuronal cells and increased expression of cytoplasmic phospholipase A(2 )was associated with increased production of prostaglandin E(2 )in response to amyloid-β(1-42 )or HuPrP82-146. Such observations suggest that IFN-γ produced within the brain may increase neuronal loss in Alzheimer's disease.