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Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression

BACKGROUND: We have studied alterations of gene expression associated with naturally-occurring early life stage mortality (M74) in Baltic salmon using a cDNA microarray and real time PCR. M74-affected fry have several typical neurological, cardiovascular and pathological symptoms. They are also char...

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Autores principales: Vuori, Kristiina AM, Koskinen, Heikki, Krasnov, Aleksei, Koivumäki, Paula, Afanasyev, Sergey, Vuorinen, Pekka J, Nikinmaa, Mikko
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435884/
https://www.ncbi.nlm.nih.gov/pubmed/16545121
http://dx.doi.org/10.1186/1471-2164-7-56
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author Vuori, Kristiina AM
Koskinen, Heikki
Krasnov, Aleksei
Koivumäki, Paula
Afanasyev, Sergey
Vuorinen, Pekka J
Nikinmaa, Mikko
author_facet Vuori, Kristiina AM
Koskinen, Heikki
Krasnov, Aleksei
Koivumäki, Paula
Afanasyev, Sergey
Vuorinen, Pekka J
Nikinmaa, Mikko
author_sort Vuori, Kristiina AM
collection PubMed
description BACKGROUND: We have studied alterations of gene expression associated with naturally-occurring early life stage mortality (M74) in Baltic salmon using a cDNA microarray and real time PCR. M74-affected fry have several typical neurological, cardiovascular and pathological symptoms. They are also characterized by low thiamine content and show signs of oxidative stress. RESULTS: Affected fry can be divided into three major groups with early, intermediate or late onset of mortality. If mortality starts during the first third of the yolk-sac stage, virtually all the responses are compatible with stress, which rapidly leads to the common terminal responses. If death occurs during the second third of the yolk sac stage, the terminal stage is preceded by a decrease in globin gene expression, which leads to internal hypoxia when the animals grow and shift from skin- to gill-breathing. Fry will eventually proceed to the terminal responses. The group developing M74 most slowly appears to compensate for reduced oxygen delivery by downregulation of metabolism, and hence some fry can escape death. CONCLUSION: Our study is the first demonstration of diverse transcriptional responses to a naturally-occurring developmental disturbance. Since many of the genes differentially expressed in M74-fry are evolutionarily conserved, the M74 of Baltic salmon can serve as a model for developmental disturbances and environmental stress responses in vertebrates in general.
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spelling pubmed-14358842006-04-14 Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression Vuori, Kristiina AM Koskinen, Heikki Krasnov, Aleksei Koivumäki, Paula Afanasyev, Sergey Vuorinen, Pekka J Nikinmaa, Mikko BMC Genomics Research Article BACKGROUND: We have studied alterations of gene expression associated with naturally-occurring early life stage mortality (M74) in Baltic salmon using a cDNA microarray and real time PCR. M74-affected fry have several typical neurological, cardiovascular and pathological symptoms. They are also characterized by low thiamine content and show signs of oxidative stress. RESULTS: Affected fry can be divided into three major groups with early, intermediate or late onset of mortality. If mortality starts during the first third of the yolk-sac stage, virtually all the responses are compatible with stress, which rapidly leads to the common terminal responses. If death occurs during the second third of the yolk sac stage, the terminal stage is preceded by a decrease in globin gene expression, which leads to internal hypoxia when the animals grow and shift from skin- to gill-breathing. Fry will eventually proceed to the terminal responses. The group developing M74 most slowly appears to compensate for reduced oxygen delivery by downregulation of metabolism, and hence some fry can escape death. CONCLUSION: Our study is the first demonstration of diverse transcriptional responses to a naturally-occurring developmental disturbance. Since many of the genes differentially expressed in M74-fry are evolutionarily conserved, the M74 of Baltic salmon can serve as a model for developmental disturbances and environmental stress responses in vertebrates in general. BioMed Central 2006-03-17 /pmc/articles/PMC1435884/ /pubmed/16545121 http://dx.doi.org/10.1186/1471-2164-7-56 Text en Copyright © 2006 Vuori et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Vuori, Kristiina AM
Koskinen, Heikki
Krasnov, Aleksei
Koivumäki, Paula
Afanasyev, Sergey
Vuorinen, Pekka J
Nikinmaa, Mikko
Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title_full Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title_fullStr Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title_full_unstemmed Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title_short Developmental disturbances in early life stage mortality (M74) of Baltic salmon fry as studied by changes in gene expression
title_sort developmental disturbances in early life stage mortality (m74) of baltic salmon fry as studied by changes in gene expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435884/
https://www.ncbi.nlm.nih.gov/pubmed/16545121
http://dx.doi.org/10.1186/1471-2164-7-56
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