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Specific RNA binding to ordered phospholipid bilayers
We have studied RNA binding to vesicles bounded by ordered and disordered phospholipid membranes. A positive correlation exists between bilayer order and RNA affinity. In particular, structure-dependent RNA binding appears for rafted (liquid-ordered) domains in sphingomyelin-cholesterol-1,2-dioleoyl...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449910/ https://www.ncbi.nlm.nih.gov/pubmed/16641318 http://dx.doi.org/10.1093/nar/gkl220 |
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author | Janas, Tadeusz Janas, Teresa Yarus, Michael |
author_facet | Janas, Tadeusz Janas, Teresa Yarus, Michael |
author_sort | Janas, Tadeusz |
collection | PubMed |
description | We have studied RNA binding to vesicles bounded by ordered and disordered phospholipid membranes. A positive correlation exists between bilayer order and RNA affinity. In particular, structure-dependent RNA binding appears for rafted (liquid-ordered) domains in sphingomyelin-cholesterol-1,2-dioleoyl-sn-glycero-3-phosphocholine vesicles. Binding to more highly ordered gel phase membranes is stronger, but much less RNA structure-dependent. All modes of RNA-membrane association seem to be electrostatic and headgroup directed. Fluorometry on 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomes indicates that bound RNA broadens the gel-fluid melting transition, and reduces lipid headgroup order, as detected via fluorometric measurement of intramembrane electric fields. RNA preference for rafted lipid was visualized and confirmed using multiple fluorophores that allow fluorescence and fluorescence resonance energy transfer microscopy on RNA molecules closely associated with ordered lipid patches within giant vesicles. Accordingly, both RNA structure and membrane order could modulate biological RNA–membrane interactions. |
format | Text |
id | pubmed-1449910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-14499102006-05-04 Specific RNA binding to ordered phospholipid bilayers Janas, Tadeusz Janas, Teresa Yarus, Michael Nucleic Acids Res Article We have studied RNA binding to vesicles bounded by ordered and disordered phospholipid membranes. A positive correlation exists between bilayer order and RNA affinity. In particular, structure-dependent RNA binding appears for rafted (liquid-ordered) domains in sphingomyelin-cholesterol-1,2-dioleoyl-sn-glycero-3-phosphocholine vesicles. Binding to more highly ordered gel phase membranes is stronger, but much less RNA structure-dependent. All modes of RNA-membrane association seem to be electrostatic and headgroup directed. Fluorometry on 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomes indicates that bound RNA broadens the gel-fluid melting transition, and reduces lipid headgroup order, as detected via fluorometric measurement of intramembrane electric fields. RNA preference for rafted lipid was visualized and confirmed using multiple fluorophores that allow fluorescence and fluorescence resonance energy transfer microscopy on RNA molecules closely associated with ordered lipid patches within giant vesicles. Accordingly, both RNA structure and membrane order could modulate biological RNA–membrane interactions. Oxford University Press 2006 2006-04-26 /pmc/articles/PMC1449910/ /pubmed/16641318 http://dx.doi.org/10.1093/nar/gkl220 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Janas, Tadeusz Janas, Teresa Yarus, Michael Specific RNA binding to ordered phospholipid bilayers |
title | Specific RNA binding to ordered phospholipid bilayers |
title_full | Specific RNA binding to ordered phospholipid bilayers |
title_fullStr | Specific RNA binding to ordered phospholipid bilayers |
title_full_unstemmed | Specific RNA binding to ordered phospholipid bilayers |
title_short | Specific RNA binding to ordered phospholipid bilayers |
title_sort | specific rna binding to ordered phospholipid bilayers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449910/ https://www.ncbi.nlm.nih.gov/pubmed/16641318 http://dx.doi.org/10.1093/nar/gkl220 |
work_keys_str_mv | AT janastadeusz specificrnabindingtoorderedphospholipidbilayers AT janasteresa specificrnabindingtoorderedphospholipidbilayers AT yarusmichael specificrnabindingtoorderedphospholipidbilayers |