Cargando…

Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle

BACKGROUND: Intergenic splicing resulting in the combination of mRNAs sequences from distinct genes is a newly identified mechanism likely to contribute to protein diversity. Few cases have been described, most of them involving neighboring genes and thus suggesting a cotranscription event presumabl...

Descripción completa

Detalles Bibliográficos
Autores principales: Roux, Matthieu, Levéziel, Hubert, Amarger, Valérie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450281/
https://www.ncbi.nlm.nih.gov/pubmed/16595010
http://dx.doi.org/10.1186/1471-2164-7-71
_version_ 1782127387344896000
author Roux, Matthieu
Levéziel, Hubert
Amarger, Valérie
author_facet Roux, Matthieu
Levéziel, Hubert
Amarger, Valérie
author_sort Roux, Matthieu
collection PubMed
description BACKGROUND: Intergenic splicing resulting in the combination of mRNAs sequences from distinct genes is a newly identified mechanism likely to contribute to protein diversity. Few cases have been described, most of them involving neighboring genes and thus suggesting a cotranscription event presumably due to transcriptional termination bypass. RESULTS: We identified bovine chimeric transcripts resulting from cotranscription and intergenic splicing of two neighboring genes, PPARG and TSEN2. These two genes encode the Peroxisome Proliferator Activated Receptors γ1 and γ2 and the tRNA Splicing Endonuclease 2 homolog and are situated in the same orientation about 50 kb apart on bovine chromosome 22q24. Their relative position is conserved in human and mouse. We identified two types of chimeric transcripts containing all but the last exon of the PPARG gene followed by all but the first exon of the TSEN2 gene. The two chimers differ by the presence/absence of an intermediate exon resulting from transcription of a LINE L2 sequence situated between the two genes. Both transcripts use canonical splice sites for all exons coming from both genes, as well as for the LINE L2 sequence. One of these transcripts harbors a premature STOP codon and the other encodes a putative chimeric protein combining most of the PPARγ protein and the entire TSEN2 protein, but we could not establish the existence of this protein. CONCLUSION: By showing that both individual and chimeric transcripts are transcribed from PPARG and TSEN2, we demonstrated regulation of transcription termination. Further, the existence and functionality of a chimeric protein harboring active motifs that are a priori unrelated is hypothesized.
format Text
id pubmed-1450281
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-14502812006-04-29 Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle Roux, Matthieu Levéziel, Hubert Amarger, Valérie BMC Genomics Research Article BACKGROUND: Intergenic splicing resulting in the combination of mRNAs sequences from distinct genes is a newly identified mechanism likely to contribute to protein diversity. Few cases have been described, most of them involving neighboring genes and thus suggesting a cotranscription event presumably due to transcriptional termination bypass. RESULTS: We identified bovine chimeric transcripts resulting from cotranscription and intergenic splicing of two neighboring genes, PPARG and TSEN2. These two genes encode the Peroxisome Proliferator Activated Receptors γ1 and γ2 and the tRNA Splicing Endonuclease 2 homolog and are situated in the same orientation about 50 kb apart on bovine chromosome 22q24. Their relative position is conserved in human and mouse. We identified two types of chimeric transcripts containing all but the last exon of the PPARG gene followed by all but the first exon of the TSEN2 gene. The two chimers differ by the presence/absence of an intermediate exon resulting from transcription of a LINE L2 sequence situated between the two genes. Both transcripts use canonical splice sites for all exons coming from both genes, as well as for the LINE L2 sequence. One of these transcripts harbors a premature STOP codon and the other encodes a putative chimeric protein combining most of the PPARγ protein and the entire TSEN2 protein, but we could not establish the existence of this protein. CONCLUSION: By showing that both individual and chimeric transcripts are transcribed from PPARG and TSEN2, we demonstrated regulation of transcription termination. Further, the existence and functionality of a chimeric protein harboring active motifs that are a priori unrelated is hypothesized. BioMed Central 2006-04-04 /pmc/articles/PMC1450281/ /pubmed/16595010 http://dx.doi.org/10.1186/1471-2164-7-71 Text en Copyright © 2006 Roux et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Roux, Matthieu
Levéziel, Hubert
Amarger, Valérie
Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title_full Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title_fullStr Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title_full_unstemmed Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title_short Cotranscription and intergenic splicing of the PPARG and TSEN2 genes in cattle
title_sort cotranscription and intergenic splicing of the pparg and tsen2 genes in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450281/
https://www.ncbi.nlm.nih.gov/pubmed/16595010
http://dx.doi.org/10.1186/1471-2164-7-71
work_keys_str_mv AT rouxmatthieu cotranscriptionandintergenicsplicingoftheppargandtsen2genesincattle
AT levezielhubert cotranscriptionandintergenicsplicingoftheppargandtsen2genesincattle
AT amargervalerie cotranscriptionandintergenicsplicingoftheppargandtsen2genesincattle