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Deoxyribozymes that recode sequence information

Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors....

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Detalles Bibliográficos
Autores principales: Tabor, Jeffrey J., Levy, Matthew, Ellington, Andrew D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450334/
https://www.ncbi.nlm.nih.gov/pubmed/16648360
http://dx.doi.org/10.1093/nar/gkl176
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author Tabor, Jeffrey J.
Levy, Matthew
Ellington, Andrew D.
author_facet Tabor, Jeffrey J.
Levy, Matthew
Ellington, Andrew D.
author_sort Tabor, Jeffrey J.
collection PubMed
description Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered ligases can ‘read’ one sequence and then ‘write’ (by ligation) a separate, distinct sequence, which can in turn be uniquely amplified. The binary deoxyribozymes show great specificity, can discriminate against a small number of mutations in the effector, and can read and recode DNA information with high fidelity even in the presence of excess obscuring genomic DNA. In addition, the binary deoxyribozymes can read non-natural nucleotides and write natural sequence information. The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs.
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spelling pubmed-14503342006-05-12 Deoxyribozymes that recode sequence information Tabor, Jeffrey J. Levy, Matthew Ellington, Andrew D. Nucleic Acids Res Article Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered ligases can ‘read’ one sequence and then ‘write’ (by ligation) a separate, distinct sequence, which can in turn be uniquely amplified. The binary deoxyribozymes show great specificity, can discriminate against a small number of mutations in the effector, and can read and recode DNA information with high fidelity even in the presence of excess obscuring genomic DNA. In addition, the binary deoxyribozymes can read non-natural nucleotides and write natural sequence information. The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs. Oxford University Press 2006 2006-04-28 /pmc/articles/PMC1450334/ /pubmed/16648360 http://dx.doi.org/10.1093/nar/gkl176 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Tabor, Jeffrey J.
Levy, Matthew
Ellington, Andrew D.
Deoxyribozymes that recode sequence information
title Deoxyribozymes that recode sequence information
title_full Deoxyribozymes that recode sequence information
title_fullStr Deoxyribozymes that recode sequence information
title_full_unstemmed Deoxyribozymes that recode sequence information
title_short Deoxyribozymes that recode sequence information
title_sort deoxyribozymes that recode sequence information
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450334/
https://www.ncbi.nlm.nih.gov/pubmed/16648360
http://dx.doi.org/10.1093/nar/gkl176
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