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Deoxyribozymes that recode sequence information
Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors....
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450334/ https://www.ncbi.nlm.nih.gov/pubmed/16648360 http://dx.doi.org/10.1093/nar/gkl176 |
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author | Tabor, Jeffrey J. Levy, Matthew Ellington, Andrew D. |
author_facet | Tabor, Jeffrey J. Levy, Matthew Ellington, Andrew D. |
author_sort | Tabor, Jeffrey J. |
collection | PubMed |
description | Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered ligases can ‘read’ one sequence and then ‘write’ (by ligation) a separate, distinct sequence, which can in turn be uniquely amplified. The binary deoxyribozymes show great specificity, can discriminate against a small number of mutations in the effector, and can read and recode DNA information with high fidelity even in the presence of excess obscuring genomic DNA. In addition, the binary deoxyribozymes can read non-natural nucleotides and write natural sequence information. The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs. |
format | Text |
id | pubmed-1450334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-14503342006-05-12 Deoxyribozymes that recode sequence information Tabor, Jeffrey J. Levy, Matthew Ellington, Andrew D. Nucleic Acids Res Article Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered ligases can ‘read’ one sequence and then ‘write’ (by ligation) a separate, distinct sequence, which can in turn be uniquely amplified. The binary deoxyribozymes show great specificity, can discriminate against a small number of mutations in the effector, and can read and recode DNA information with high fidelity even in the presence of excess obscuring genomic DNA. In addition, the binary deoxyribozymes can read non-natural nucleotides and write natural sequence information. The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs. Oxford University Press 2006 2006-04-28 /pmc/articles/PMC1450334/ /pubmed/16648360 http://dx.doi.org/10.1093/nar/gkl176 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Tabor, Jeffrey J. Levy, Matthew Ellington, Andrew D. Deoxyribozymes that recode sequence information |
title | Deoxyribozymes that recode sequence information |
title_full | Deoxyribozymes that recode sequence information |
title_fullStr | Deoxyribozymes that recode sequence information |
title_full_unstemmed | Deoxyribozymes that recode sequence information |
title_short | Deoxyribozymes that recode sequence information |
title_sort | deoxyribozymes that recode sequence information |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450334/ https://www.ncbi.nlm.nih.gov/pubmed/16648360 http://dx.doi.org/10.1093/nar/gkl176 |
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