Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy

BACKGROUND: The establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in...

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Autores principales: Cimino, Mena, Alamo, Lorenzo, Salazar, Leiria
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458343/
https://www.ncbi.nlm.nih.gov/pubmed/16620389
http://dx.doi.org/10.1186/1471-2180-6-35
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author Cimino, Mena
Alamo, Lorenzo
Salazar, Leiria
author_facet Cimino, Mena
Alamo, Lorenzo
Salazar, Leiria
author_sort Cimino, Mena
collection PubMed
description BACKGROUND: The establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in effectively permeabilize it. RESULTS: A treatment combining lysozyme with triton X-100 was found to be an effective permeabilization method of the mycobacterial envelope. CONCLUSION: A rapid and simple permeabilization protocol has been successfully assessed in pure cultures of both Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. This method can be successful used in the cytolocalization of proteins by immunolabeling.
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spelling pubmed-14583432006-05-06 Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy Cimino, Mena Alamo, Lorenzo Salazar, Leiria BMC Microbiol Methodology Article BACKGROUND: The establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in effectively permeabilize it. RESULTS: A treatment combining lysozyme with triton X-100 was found to be an effective permeabilization method of the mycobacterial envelope. CONCLUSION: A rapid and simple permeabilization protocol has been successfully assessed in pure cultures of both Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. This method can be successful used in the cytolocalization of proteins by immunolabeling. BioMed Central 2006-04-18 /pmc/articles/PMC1458343/ /pubmed/16620389 http://dx.doi.org/10.1186/1471-2180-6-35 Text en Copyright © 2006 Cimino et al; licensee BioMed Central Ltd.
spellingShingle Methodology Article
Cimino, Mena
Alamo, Lorenzo
Salazar, Leiria
Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title_full Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title_fullStr Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title_full_unstemmed Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title_short Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
title_sort permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458343/
https://www.ncbi.nlm.nih.gov/pubmed/16620389
http://dx.doi.org/10.1186/1471-2180-6-35
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AT alamolorenzo permeabilizationofthemycobacterialenvelopeforproteincytolocalizationstudiesbyimmunofluorescencemicroscopy
AT salazarleiria permeabilizationofthemycobacterialenvelopeforproteincytolocalizationstudiesbyimmunofluorescencemicroscopy

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