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A SINE in the genome of the cephalochordate amphioxus is an Alu element

Transposable elements of about 300 bp, termed “short interspersed nucleotide elements or SINEs are common in eukaryotes. However, Alu elements, SINEs containing restriction sites for the AluI enzyme, have been known only from primates. Here I report the first SINE found in the genome of the cephaloc...

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Autor principal: Holland, Linda Z.
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458429/
https://www.ncbi.nlm.nih.gov/pubmed/16733535
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author Holland, Linda Z.
author_facet Holland, Linda Z.
author_sort Holland, Linda Z.
collection PubMed
description Transposable elements of about 300 bp, termed “short interspersed nucleotide elements or SINEs are common in eukaryotes. However, Alu elements, SINEs containing restriction sites for the AluI enzyme, have been known only from primates. Here I report the first SINE found in the genome of the cephalochordate, amphioxus. It is an Alu element of 375 bp that does not share substantial identity with any genomic sequences in vertebrates. It was identified because it was located in the FoxD regulatory region in a cosmid derived from one individual, but absent from the two FoxD alleles of BACs from a second individual. However, searches of sequences of BACs and genomic traces from this second individual gave an estimate of 50-100 copies in the amphioxus genome. The finding of an Alu element in amphioxus raises the question of whether Alu elements in amphioxus and primates arose by convergent evolution or by inheritance from a common ancestor. Genome-wide analyses of transposable elements in amphioxus and other chordates such as tunicates, agnathans and cartilaginous fishes could well provide the answer.
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spelling pubmed-14584292006-05-26 A SINE in the genome of the cephalochordate amphioxus is an Alu element Holland, Linda Z. Int J Biol Sci Research Paper Transposable elements of about 300 bp, termed “short interspersed nucleotide elements or SINEs are common in eukaryotes. However, Alu elements, SINEs containing restriction sites for the AluI enzyme, have been known only from primates. Here I report the first SINE found in the genome of the cephalochordate, amphioxus. It is an Alu element of 375 bp that does not share substantial identity with any genomic sequences in vertebrates. It was identified because it was located in the FoxD regulatory region in a cosmid derived from one individual, but absent from the two FoxD alleles of BACs from a second individual. However, searches of sequences of BACs and genomic traces from this second individual gave an estimate of 50-100 copies in the amphioxus genome. The finding of an Alu element in amphioxus raises the question of whether Alu elements in amphioxus and primates arose by convergent evolution or by inheritance from a common ancestor. Genome-wide analyses of transposable elements in amphioxus and other chordates such as tunicates, agnathans and cartilaginous fishes could well provide the answer. Ivyspring International Publisher 2006-04-10 /pmc/articles/PMC1458429/ /pubmed/16733535 Text en © Ivyspring International Publisher. This is an open access article. Reproduction is permitted for personal and noncommerical use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Holland, Linda Z.
A SINE in the genome of the cephalochordate amphioxus is an Alu element
title A SINE in the genome of the cephalochordate amphioxus is an Alu element
title_full A SINE in the genome of the cephalochordate amphioxus is an Alu element
title_fullStr A SINE in the genome of the cephalochordate amphioxus is an Alu element
title_full_unstemmed A SINE in the genome of the cephalochordate amphioxus is an Alu element
title_short A SINE in the genome of the cephalochordate amphioxus is an Alu element
title_sort sine in the genome of the cephalochordate amphioxus is an alu element
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458429/
https://www.ncbi.nlm.nih.gov/pubmed/16733535
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