Cargando…

Phage display mediated immuno-PCR

Immuno-PCR (IPCR) is a powerful detection technology in immunological study and clinical diagnosis due to its ultrasensitivity. Here we introduce a new strategy termed phage display mediated immuno-PCR (PD-IPCR). Instead of utilization of monoclonal antibody (mAb) and chemically bond DNA that requir...

Descripción completa

Detalles Bibliográficos
Autores principales:	Guo, Yong-Chao, Zhou, Ya-Feng, Zhang, Xian-En, Zhang, Zhi-Ping, Qiao, Yan-Mei, Bi, Li-Jun, Wen, Ji-Kai, Liang, Mi-Fang, Zhang, Ji-Bin
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458518/ https://www.ncbi.nlm.nih.gov/pubmed/16682441 http://dx.doi.org/10.1093/nar/gkl260

Ejemplares similares

Eliminating helper phage from phage display
por: Chasteen, L., et al.
Publicado: (2006)

Fluorescent T7 display phages obtained by translational frameshift
por: Slootweg, Erik J., et al.
Publicado: (2006)

DeltaPhage—a novel helper phage for high-valence pIX phagemid display
por: Nilssen, Nicolay R., et al.
Publicado: (2012)

Phage display peptide libraries: deviations from randomness and correctives
por: Ryvkin, Arie, et al.
Publicado: (2018)

Tandem phage-display for the identification of non-overlapping binding pairs of recombinant affinity reagents
por: Gorman, Kevin T., et al.
Publicado: (2017)

One-step zero-background IgG reformatting of phage-displayed antibody fragments enabling rapid and high-throughput lead identification
por: Chen, Chao-Guang, et al.
Publicado: (2014)

A comprehensive analysis of filamentous phage display vectors for cytoplasmic proteins: an analysis with different fluorescent proteins
por: Velappan, Nileena, et al.
Publicado: (2010)

Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library
por: Schoonbroodt, Sonia, et al.
Publicado: (2005)

SiteFinding-PCR: a simple and efficient PCR method for chromosome walking
por: Tan, Guihong, et al.
Publicado: (2005)

Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
por: Ding, Ruihua, et al.
Publicado: (2023)

A novel method for generating and screening peptides and libraries displayed on adenovirus fiber
por: Lupold, Shawn E., et al.
Publicado: (2007)

Enhancing the efficiency of a PCR using gold nanoparticles
por: Li, Min, et al.
Publicado: (2005)

Bicistronic DNA display for in vitro selection of Fab fragments
por: Sumida, Takeshi, et al.
Publicado: (2009)

Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon
por: Gondai, Takuma, et al.
Publicado: (2008)

Miniature RT–PCR system for diagnosis of RNA-based viruses
por: Liao, Chia-Sheng, et al.
Publicado: (2005)

Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations
por: Liu, Qian, et al.
Publicado: (2021)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Selection of functional human antibodies from retroviral display libraries
por: Urban, Johannes H., et al.
Publicado: (2005)

Application of phage display to high throughput antibody generation and characterization
por: Schofield, Darren J, et al.
Publicado: (2007)

Direct selection and phage display of a Gram-positive secretome
por: Jankovic, Dragana, et al.
Publicado: (2007)

Identification of target-binding peptide motifs by high-throughput sequencing of phage-selected peptides
por: Rentero Rebollo, Inmaculada, et al.
Publicado: (2014)

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
por: Wu, Wu, et al.
Publicado: (2005)

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
por: Lebedev, Alexandre V., et al.
Publicado: (2008)

In vitro evolution of single-chain antibodies using mRNA display
por: Fukuda, Isao, et al.
Publicado: (2006)

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
por: Khodakaramian, Gholam, et al.
Publicado: (2006)

A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda
por: Zanghi, Christine N., et al.
Publicado: (2005)

Covalent antibody display—an in vitro antibody-DNA library selection system
por: Reiersen, Herald, et al.
Publicado: (2005)

Rapid antibody selection by mRNA display on a microfluidic chip
por: Tabata, Noriko, et al.
Publicado: (2009)

Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes
por: Neuzil, Pavel, et al.
Publicado: (2006)

Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
por: Lievens, Antoon, et al.
Publicado: (2012)

Nanoliter high throughput quantitative PCR
por: Morrison, Tom, et al.
Publicado: (2006)

Real-time quantification of microRNAs by stem–loop RT–PCR
por: Chen, Caifu, et al.
Publicado: (2005)

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
por: Shaik, Gouse M., et al.
Publicado: (2008)

Next-generation sequencing-guided identification and reconstruction of antibody CDR combinations from phage selection outputs
por: Barreto, Kris, et al.
Publicado: (2019)

Affinity selection of DNA-binding protein complexes using mRNA display
por: Tateyama, Seiji, et al.
Publicado: (2006)

Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins
por: Hughes, Marcus D., et al.
Publicado: (2005)

Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products
por: Shigemori, Yasushi, et al.
Publicado: (2005)

Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection
por: Liao, Yiqun, et al.
Publicado: (2013)

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR
por: Shuga, Joe, et al.
Publicado: (2013)

Predicting failure rate of PCR in large genomes
por: Andreson, Reidar, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_jkr0k8m8rqkabshqgacc2kih6e