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Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation

Chromatin-modifying factors play key roles in transcription, DNA replication and DNA repair. Post-translational modification of these proteins is largely responsible for regulating their activity. The FACT (facilitates chromatin transcription) complex, a heterodimer of hSpt16 and SSRP1, is a chromat...

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Autores principales: Huang, Jing-Yi, Chen, Wei-Hao, Chang, Ya-Ling, Wang, Hsiao-Ting, Chuang, Wan-ting, Lee, Sheng-Chung
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458519/
https://www.ncbi.nlm.nih.gov/pubmed/16682447
http://dx.doi.org/10.1093/nar/gkl241
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author Huang, Jing-Yi
Chen, Wei-Hao
Chang, Ya-Ling
Wang, Hsiao-Ting
Chuang, Wan-ting
Lee, Sheng-Chung
author_facet Huang, Jing-Yi
Chen, Wei-Hao
Chang, Ya-Ling
Wang, Hsiao-Ting
Chuang, Wan-ting
Lee, Sheng-Chung
author_sort Huang, Jing-Yi
collection PubMed
description Chromatin-modifying factors play key roles in transcription, DNA replication and DNA repair. Post-translational modification of these proteins is largely responsible for regulating their activity. The FACT (facilitates chromatin transcription) complex, a heterodimer of hSpt16 and SSRP1, is a chromatin structure modulator whose involvement in transcription and DNA replication has been reported. Here we show that nucleosome binding activity of FACT complex is regulated by poly(ADP-ribosyl)ation. hSpt16, the large subunit of FACT, is poly(ADP-ribosyl)ated by poly(ADP-ribose) polymerase-1 (PARP-1) resulting from physical interaction between these two proteins. The level of hSpt16 poly(ADP-ribosyl)ation is elevated after genotoxic treatment and coincides with the activation of PARP-1. The enhanced hSpt16 poly(ADP-ribosyl)ation level correlates with the dissociation of FACT from chromatin in response to DNA damage. Our findings suggest that poly(ADP-ribosyl)ation of hSpt16 by PARP-1 play regulatory roles for FACT-mediated chromatin remodeling.
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spelling pubmed-14585192006-05-12 Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation Huang, Jing-Yi Chen, Wei-Hao Chang, Ya-Ling Wang, Hsiao-Ting Chuang, Wan-ting Lee, Sheng-Chung Nucleic Acids Res Article Chromatin-modifying factors play key roles in transcription, DNA replication and DNA repair. Post-translational modification of these proteins is largely responsible for regulating their activity. The FACT (facilitates chromatin transcription) complex, a heterodimer of hSpt16 and SSRP1, is a chromatin structure modulator whose involvement in transcription and DNA replication has been reported. Here we show that nucleosome binding activity of FACT complex is regulated by poly(ADP-ribosyl)ation. hSpt16, the large subunit of FACT, is poly(ADP-ribosyl)ated by poly(ADP-ribose) polymerase-1 (PARP-1) resulting from physical interaction between these two proteins. The level of hSpt16 poly(ADP-ribosyl)ation is elevated after genotoxic treatment and coincides with the activation of PARP-1. The enhanced hSpt16 poly(ADP-ribosyl)ation level correlates with the dissociation of FACT from chromatin in response to DNA damage. Our findings suggest that poly(ADP-ribosyl)ation of hSpt16 by PARP-1 play regulatory roles for FACT-mediated chromatin remodeling. Oxford University Press 2006 2006-05-08 /pmc/articles/PMC1458519/ /pubmed/16682447 http://dx.doi.org/10.1093/nar/gkl241 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Huang, Jing-Yi
Chen, Wei-Hao
Chang, Ya-Ling
Wang, Hsiao-Ting
Chuang, Wan-ting
Lee, Sheng-Chung
Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title_full Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title_fullStr Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title_full_unstemmed Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title_short Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
title_sort modulation of nucleosome-binding activity of fact by poly(adp-ribosyl)ation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458519/
https://www.ncbi.nlm.nih.gov/pubmed/16682447
http://dx.doi.org/10.1093/nar/gkl241
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