Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.

The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation....

Descripción completa

Detalles Bibliográficos
Autores principales: Zaichkina, S I, Aptikaeva, G F, Rozanova, O M, Akhmadieva, A K, Smirnova, E N, Ganassi, E E
Formato: Texto
Lenguaje:English
Publicado: 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1469940/
https://www.ncbi.nlm.nih.gov/pubmed/9467059
_version_ 1782127718232489984
author Zaichkina, S I
Aptikaeva, G F
Rozanova, O M
Akhmadieva, A K
Smirnova, E N
Ganassi, E E
author_facet Zaichkina, S I
Aptikaeva, G F
Rozanova, O M
Akhmadieva, A K
Smirnova, E N
Ganassi, E E
author_sort Zaichkina, S I
collection PubMed
description The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation. All structural chromosome- and chromatid-type changes were recorded. The experimental data showed that the conditions of irradiation of lymphocytes affected neither the background level of chromosome damage nor their radiosensitivity. The obtained dose-response curve of chromosome aberrations was described by a linear regression, which then became a plateau. There is no statistically significant difference between the results for the low doses (10-50 cGy) of chronic and acute radiation.
format Text
id pubmed-1469940
institution National Center for Biotechnology Information
language English
publishDate 1997
record_format MEDLINE/PubMed
spelling pubmed-14699402006-06-01 Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture. Zaichkina, S I Aptikaeva, G F Rozanova, O M Akhmadieva, A K Smirnova, E N Ganassi, E E Environ Health Perspect Research Article The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation. All structural chromosome- and chromatid-type changes were recorded. The experimental data showed that the conditions of irradiation of lymphocytes affected neither the background level of chromosome damage nor their radiosensitivity. The obtained dose-response curve of chromosome aberrations was described by a linear regression, which then became a plateau. There is no statistically significant difference between the results for the low doses (10-50 cGy) of chronic and acute radiation. 1997-12 /pmc/articles/PMC1469940/ /pubmed/9467059 Text en
spellingShingle Research Article
Zaichkina, S I
Aptikaeva, G F
Rozanova, O M
Akhmadieva, A K
Smirnova, E N
Ganassi, E E
Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title_full Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title_fullStr Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title_full_unstemmed Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title_short Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
title_sort action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1469940/
https://www.ncbi.nlm.nih.gov/pubmed/9467059
work_keys_str_mv AT zaichkinasi actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture
AT aptikaevagf actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture
AT rozanovaom actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture
AT akhmadievaak actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture
AT smirnovaen actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture
AT ganassiee actionofchronicirradiationonthecytogeneticdamageofhumanlymphocyteculture

Ejemplares similares