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Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.

The purpose of this study was to test the hypothesis that particulate matter < or = 10 microns in aerodynamic diameter (PM10) particles have the ability to generate free radical activity at their surface. We collected PM10 filters from the Edinburgh, United Kingdom, Enhanced Urban Network samplin...

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Detalles Bibliográficos
Autores principales: Donaldson, K, Brown, D M, Mitchell, C, Dineva, M, Beswick, P H, Gilmour, P, MacNee, W
Formato: Texto
Lenguaje:English
Publicado: 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470141/
https://www.ncbi.nlm.nih.gov/pubmed/9400739
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author Donaldson, K
Brown, D M
Mitchell, C
Dineva, M
Beswick, P H
Gilmour, P
MacNee, W
author_facet Donaldson, K
Brown, D M
Mitchell, C
Dineva, M
Beswick, P H
Gilmour, P
MacNee, W
author_sort Donaldson, K
collection PubMed
description The purpose of this study was to test the hypothesis that particulate matter < or = 10 microns in aerodynamic diameter (PM10) particles have the ability to generate free radical activity at their surface. We collected PM10 filters from the Edinburgh, United Kingdom, Enhanced Urban Network sampling site, removed particles from the filter, and tested their ability to cause free radical damage to supercoiled plasmid DNA. We found that the PM10 particles did cause damage to the DNA that was mediated by hydroxyl radicals, as shown by inhibition of the injury with mannitol. The PM10-associated hydroxyl radical activity was confirmed using a high-performance liquid chromatography-based assay to measure the hydroxyl radical adduct of salicylic acid. Desferrioxamine abolished the hydroxyl radical-mediated injury, which suggests that iron was involved. Analysis of PM10 filters confirmed the presence of large amounts of iron and leaching studies confirmed that the PM10 samples could release substantial amounts of Fe(III) and lesser amounts of Fe(II). To investigate the size of the particles involved in the hydroxyl radical injury, we centrifuged the suspension of PM10 to clarity, tested the clear supernatant, and found that it had all of the suspension activity. We conclude, therefore, that the free radical activity is derived either from a fraction that is not centrifugeable on a bench centrifuge, or that the radical generating system is released into solution.
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spelling pubmed-14701412006-06-01 Free radical activity of PM10: iron-mediated generation of hydroxyl radicals. Donaldson, K Brown, D M Mitchell, C Dineva, M Beswick, P H Gilmour, P MacNee, W Environ Health Perspect Research Article The purpose of this study was to test the hypothesis that particulate matter < or = 10 microns in aerodynamic diameter (PM10) particles have the ability to generate free radical activity at their surface. We collected PM10 filters from the Edinburgh, United Kingdom, Enhanced Urban Network sampling site, removed particles from the filter, and tested their ability to cause free radical damage to supercoiled plasmid DNA. We found that the PM10 particles did cause damage to the DNA that was mediated by hydroxyl radicals, as shown by inhibition of the injury with mannitol. The PM10-associated hydroxyl radical activity was confirmed using a high-performance liquid chromatography-based assay to measure the hydroxyl radical adduct of salicylic acid. Desferrioxamine abolished the hydroxyl radical-mediated injury, which suggests that iron was involved. Analysis of PM10 filters confirmed the presence of large amounts of iron and leaching studies confirmed that the PM10 samples could release substantial amounts of Fe(III) and lesser amounts of Fe(II). To investigate the size of the particles involved in the hydroxyl radical injury, we centrifuged the suspension of PM10 to clarity, tested the clear supernatant, and found that it had all of the suspension activity. We conclude, therefore, that the free radical activity is derived either from a fraction that is not centrifugeable on a bench centrifuge, or that the radical generating system is released into solution. 1997-09 /pmc/articles/PMC1470141/ /pubmed/9400739 Text en
spellingShingle Research Article
Donaldson, K
Brown, D M
Mitchell, C
Dineva, M
Beswick, P H
Gilmour, P
MacNee, W
Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title_full Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title_fullStr Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title_full_unstemmed Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title_short Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.
title_sort free radical activity of pm10: iron-mediated generation of hydroxyl radicals.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470141/
https://www.ncbi.nlm.nih.gov/pubmed/9400739
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