Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos pl...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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1997
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470167/ https://www.ncbi.nlm.nih.gov/pubmed/9400708 |
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author | Rahman, Q Mahmood, N Khan, S G Arif, J M Athar, M |
author_facet | Rahman, Q Mahmood, N Khan, S G Arif, J M Athar, M |
author_sort | Rahman, Q |
collection | PubMed |
description | Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos plays an important role in the asbestos-mediated generation of reactive oxygen species. Exposure to asbestos leads to degradation of heme proteins such as cytochrome P450-releasing heme in cytosol. Our simulation experiments in the presence of heme show that such asbestos-released heme may increase lipid peroxidation and can cause DNA damage. Further, heme and horseradish peroxidase (HRP) can cause extensive DNA damage in the presence of asbestos and hydrogen peroxide/organic peroxide/hydroperoxides. HRP catalyzes oxidation reactions in a manner similar to that of prostaglandin H synthetase. Iron released from asbestos is only partially responsible for DNA damage. However, our studies indicate that DNA damage mediated by asbestos in vivo may be caused by a combination of effects such as the release and participation of iron, heme, and heme moiety of prostaglandin H synthetase in free radical generation from peroxides and hydroperoxides. |
format | Text |
id | pubmed-1470167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
record_format | MEDLINE/PubMed |
spelling | pubmed-14701672006-06-01 Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. Rahman, Q Mahmood, N Khan, S G Arif, J M Athar, M Environ Health Perspect Research Article Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos plays an important role in the asbestos-mediated generation of reactive oxygen species. Exposure to asbestos leads to degradation of heme proteins such as cytochrome P450-releasing heme in cytosol. Our simulation experiments in the presence of heme show that such asbestos-released heme may increase lipid peroxidation and can cause DNA damage. Further, heme and horseradish peroxidase (HRP) can cause extensive DNA damage in the presence of asbestos and hydrogen peroxide/organic peroxide/hydroperoxides. HRP catalyzes oxidation reactions in a manner similar to that of prostaglandin H synthetase. Iron released from asbestos is only partially responsible for DNA damage. However, our studies indicate that DNA damage mediated by asbestos in vivo may be caused by a combination of effects such as the release and participation of iron, heme, and heme moiety of prostaglandin H synthetase in free radical generation from peroxides and hydroperoxides. 1997-09 /pmc/articles/PMC1470167/ /pubmed/9400708 Text en |
spellingShingle | Research Article Rahman, Q Mahmood, N Khan, S G Arif, J M Athar, M Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title | Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title_full | Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title_fullStr | Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title_full_unstemmed | Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title_short | Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. |
title_sort | mechanism of asbestos-mediated dna damage: role of heme and heme proteins. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470167/ https://www.ncbi.nlm.nih.gov/pubmed/9400708 |
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