Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.

Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos pl...

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Detalles Bibliográficos
Autores principales: Rahman, Q, Mahmood, N, Khan, S G, Arif, J M, Athar, M
Formato: Texto
Lenguaje:English
Publicado: 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470167/
https://www.ncbi.nlm.nih.gov/pubmed/9400708
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author Rahman, Q
Mahmood, N
Khan, S G
Arif, J M
Athar, M
author_facet Rahman, Q
Mahmood, N
Khan, S G
Arif, J M
Athar, M
author_sort Rahman, Q
collection PubMed
description Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos plays an important role in the asbestos-mediated generation of reactive oxygen species. Exposure to asbestos leads to degradation of heme proteins such as cytochrome P450-releasing heme in cytosol. Our simulation experiments in the presence of heme show that such asbestos-released heme may increase lipid peroxidation and can cause DNA damage. Further, heme and horseradish peroxidase (HRP) can cause extensive DNA damage in the presence of asbestos and hydrogen peroxide/organic peroxide/hydroperoxides. HRP catalyzes oxidation reactions in a manner similar to that of prostaglandin H synthetase. Iron released from asbestos is only partially responsible for DNA damage. However, our studies indicate that DNA damage mediated by asbestos in vivo may be caused by a combination of effects such as the release and participation of iron, heme, and heme moiety of prostaglandin H synthetase in free radical generation from peroxides and hydroperoxides.
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spelling pubmed-14701672006-06-01 Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins. Rahman, Q Mahmood, N Khan, S G Arif, J M Athar, M Environ Health Perspect Research Article Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system that may play a role in the manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated that iron associated with asbestos plays an important role in the asbestos-mediated generation of reactive oxygen species. Exposure to asbestos leads to degradation of heme proteins such as cytochrome P450-releasing heme in cytosol. Our simulation experiments in the presence of heme show that such asbestos-released heme may increase lipid peroxidation and can cause DNA damage. Further, heme and horseradish peroxidase (HRP) can cause extensive DNA damage in the presence of asbestos and hydrogen peroxide/organic peroxide/hydroperoxides. HRP catalyzes oxidation reactions in a manner similar to that of prostaglandin H synthetase. Iron released from asbestos is only partially responsible for DNA damage. However, our studies indicate that DNA damage mediated by asbestos in vivo may be caused by a combination of effects such as the release and participation of iron, heme, and heme moiety of prostaglandin H synthetase in free radical generation from peroxides and hydroperoxides. 1997-09 /pmc/articles/PMC1470167/ /pubmed/9400708 Text en
spellingShingle Research Article
Rahman, Q
Mahmood, N
Khan, S G
Arif, J M
Athar, M
Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title_full Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title_fullStr Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title_full_unstemmed Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title_short Mechanism of asbestos-mediated DNA damage: role of heme and heme proteins.
title_sort mechanism of asbestos-mediated dna damage: role of heme and heme proteins.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470167/
https://www.ncbi.nlm.nih.gov/pubmed/9400708
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