Cargando…

The estrogenic activity of phthalate esters in vitro.

A large number of phthalate esters were screened for estrogenic activity using a recombinant yeast screen. a selection of these was also tested for mitogenic effect on estrogen-responsive human breast cancer cells. A small number of the commercially available phthalates tested showed extremely weak...

Descripción completa

Detalles Bibliográficos
Autores principales: Harris, C A, Henttu, P, Parker, M G, Sumpter, J P
Formato: Texto
Lenguaje:English
Publicado: 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470189/
https://www.ncbi.nlm.nih.gov/pubmed/9347895
_version_ 1782127774625955840
author Harris, C A
Henttu, P
Parker, M G
Sumpter, J P
author_facet Harris, C A
Henttu, P
Parker, M G
Sumpter, J P
author_sort Harris, C A
collection PubMed
description A large number of phthalate esters were screened for estrogenic activity using a recombinant yeast screen. a selection of these was also tested for mitogenic effect on estrogen-responsive human breast cancer cells. A small number of the commercially available phthalates tested showed extremely weak estrogenic activity. The relative potencies of these descended in the order butyl benzyl phthalate (BBP) > dibutyl phthalate (DBP) > diisobutyl phthalate (DIBP) > diethyl phthalate (DEP) > diisiononyl phthalate (DINP). Potencies ranged from approximately 1 x 10(6) to 5 x 10(7) times less than 17beta-estradiol. The phthalates that were estrogenic in the yeast screen were also mitogenic on the human breast cancer cells. Di(2-ethylhexyl) phthalate (DEHP) showed no estrogenic activity in these in vitro assays. A number of metabolites were tested, including mono-butyl phthalate, mono-benzyl phthalate, mono-ethylhexyl phthalate, mon-n-octyl phthalate; all were wound to be inactive. One of the phthalates, ditridecyl phthalate (DTDP), produced inconsistent results; one sample was weakly estrogenic, whereas another, obtained from a different source, was inactive. analysis by gel chromatography-mass spectometry showed that the preparation exhibiting estrogenic activity contained 0.5% of the ortho-isomer of bisphenol A. It is likely that the presence of this antioxidant in the phthalate standard was responsible for the generation of a dose-response curve--which was not observed with an alternative sample that had not been supplemented with o,p'-bisphenol A--in the yeast screen; hence, DTDP is probably not weakly estrogenic. The activities of simple mixtures of BBP, DBP, and 17beta-estradiol were assessed in the yeast screen. No synergism was observed, although the activities of the mixtures were approximately additive. In summary, a small number of phthalates are weakly estrogenic in vitro. No data has yet been published on whether these are also estrogenic in vitro. No data has yet been published on whether these are also estrogenic in vivo; this will require tests using different classes of vertebrates and different routes of exposure.
format Text
id pubmed-1470189
institution National Center for Biotechnology Information
language English
publishDate 1997
record_format MEDLINE/PubMed
spelling pubmed-14701892006-06-01 The estrogenic activity of phthalate esters in vitro. Harris, C A Henttu, P Parker, M G Sumpter, J P Environ Health Perspect Research Article A large number of phthalate esters were screened for estrogenic activity using a recombinant yeast screen. a selection of these was also tested for mitogenic effect on estrogen-responsive human breast cancer cells. A small number of the commercially available phthalates tested showed extremely weak estrogenic activity. The relative potencies of these descended in the order butyl benzyl phthalate (BBP) > dibutyl phthalate (DBP) > diisobutyl phthalate (DIBP) > diethyl phthalate (DEP) > diisiononyl phthalate (DINP). Potencies ranged from approximately 1 x 10(6) to 5 x 10(7) times less than 17beta-estradiol. The phthalates that were estrogenic in the yeast screen were also mitogenic on the human breast cancer cells. Di(2-ethylhexyl) phthalate (DEHP) showed no estrogenic activity in these in vitro assays. A number of metabolites were tested, including mono-butyl phthalate, mono-benzyl phthalate, mono-ethylhexyl phthalate, mon-n-octyl phthalate; all were wound to be inactive. One of the phthalates, ditridecyl phthalate (DTDP), produced inconsistent results; one sample was weakly estrogenic, whereas another, obtained from a different source, was inactive. analysis by gel chromatography-mass spectometry showed that the preparation exhibiting estrogenic activity contained 0.5% of the ortho-isomer of bisphenol A. It is likely that the presence of this antioxidant in the phthalate standard was responsible for the generation of a dose-response curve--which was not observed with an alternative sample that had not been supplemented with o,p'-bisphenol A--in the yeast screen; hence, DTDP is probably not weakly estrogenic. The activities of simple mixtures of BBP, DBP, and 17beta-estradiol were assessed in the yeast screen. No synergism was observed, although the activities of the mixtures were approximately additive. In summary, a small number of phthalates are weakly estrogenic in vitro. No data has yet been published on whether these are also estrogenic in vitro. No data has yet been published on whether these are also estrogenic in vivo; this will require tests using different classes of vertebrates and different routes of exposure. 1997-08 /pmc/articles/PMC1470189/ /pubmed/9347895 Text en
spellingShingle Research Article
Harris, C A
Henttu, P
Parker, M G
Sumpter, J P
The estrogenic activity of phthalate esters in vitro.
title The estrogenic activity of phthalate esters in vitro.
title_full The estrogenic activity of phthalate esters in vitro.
title_fullStr The estrogenic activity of phthalate esters in vitro.
title_full_unstemmed The estrogenic activity of phthalate esters in vitro.
title_short The estrogenic activity of phthalate esters in vitro.
title_sort estrogenic activity of phthalate esters in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470189/
https://www.ncbi.nlm.nih.gov/pubmed/9347895
work_keys_str_mv AT harrisca theestrogenicactivityofphthalateestersinvitro
AT henttup theestrogenicactivityofphthalateestersinvitro
AT parkermg theestrogenicactivityofphthalateestersinvitro
AT sumpterjp theestrogenicactivityofphthalateestersinvitro
AT harrisca estrogenicactivityofphthalateestersinvitro
AT henttup estrogenicactivityofphthalateestersinvitro
AT parkermg estrogenicactivityofphthalateestersinvitro
AT sumpterjp estrogenicactivityofphthalateestersinvitro