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Chromosome XII context is important for rDNA function in yeast
The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of ∼150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474064/ https://www.ncbi.nlm.nih.gov/pubmed/16738130 http://dx.doi.org/10.1093/nar/gkl293 |
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author | Kim, Yeon-Hee Ishikawa, Daisuke Ha, Ho Phu Sugiyama, Minetaka Kaneko, Yoshinobu Harashima, Satoshi |
author_facet | Kim, Yeon-Hee Ishikawa, Daisuke Ha, Ho Phu Sugiyama, Minetaka Kaneko, Yoshinobu Harashima, Satoshi |
author_sort | Kim, Yeon-Hee |
collection | PubMed |
description | The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of ∼150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split at both sides of the rDNA cluster and strains harboring deleted variants of chromosome XII consisting of 450 kb, 1500 kb (rDNA cluster only) and 610 kb were created. In the strain harboring the 1500 kb variant of chromosome XII consisting solely of rDNA, the size of the rDNA cluster was found to decrease as a result of a decrease in rDNA copy number. The frequency of silencing of URA3 inserted within the rDNA locus was found to be greater than in a wild-type strain. The localization and morphology of the nucleolus was also affected such that a single and occasionally (6–12% frequency) two foci for Nop1p and a rounded nucleolus were observed, whereas a typical crescent-shaped nucleolar structure was seen in the wild-type strain. Notably, strains harboring the 450 kb chromosome XII variant and/or the 1500 kb variant consisting solely of rDNA had shorter life spans than wild type and also accumulated extrachromosomal rDNA circles. These observations suggest that the context of chromosome XII plays an important role in maintaining a constant rDNA copy number and in physiological processes related to rDNA function in S.cerevisiae. |
format | Text |
id | pubmed-1474064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-14740642006-06-22 Chromosome XII context is important for rDNA function in yeast Kim, Yeon-Hee Ishikawa, Daisuke Ha, Ho Phu Sugiyama, Minetaka Kaneko, Yoshinobu Harashima, Satoshi Nucleic Acids Res Article The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of ∼150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split at both sides of the rDNA cluster and strains harboring deleted variants of chromosome XII consisting of 450 kb, 1500 kb (rDNA cluster only) and 610 kb were created. In the strain harboring the 1500 kb variant of chromosome XII consisting solely of rDNA, the size of the rDNA cluster was found to decrease as a result of a decrease in rDNA copy number. The frequency of silencing of URA3 inserted within the rDNA locus was found to be greater than in a wild-type strain. The localization and morphology of the nucleolus was also affected such that a single and occasionally (6–12% frequency) two foci for Nop1p and a rounded nucleolus were observed, whereas a typical crescent-shaped nucleolar structure was seen in the wild-type strain. Notably, strains harboring the 450 kb chromosome XII variant and/or the 1500 kb variant consisting solely of rDNA had shorter life spans than wild type and also accumulated extrachromosomal rDNA circles. These observations suggest that the context of chromosome XII plays an important role in maintaining a constant rDNA copy number and in physiological processes related to rDNA function in S.cerevisiae. Oxford University Press 2006 2006-05-31 /pmc/articles/PMC1474064/ /pubmed/16738130 http://dx.doi.org/10.1093/nar/gkl293 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Kim, Yeon-Hee Ishikawa, Daisuke Ha, Ho Phu Sugiyama, Minetaka Kaneko, Yoshinobu Harashima, Satoshi Chromosome XII context is important for rDNA function in yeast |
title | Chromosome XII context is important for rDNA function in yeast |
title_full | Chromosome XII context is important for rDNA function in yeast |
title_fullStr | Chromosome XII context is important for rDNA function in yeast |
title_full_unstemmed | Chromosome XII context is important for rDNA function in yeast |
title_short | Chromosome XII context is important for rDNA function in yeast |
title_sort | chromosome xii context is important for rdna function in yeast |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474064/ https://www.ncbi.nlm.nih.gov/pubmed/16738130 http://dx.doi.org/10.1093/nar/gkl293 |
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