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Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapi...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474073/ https://www.ncbi.nlm.nih.gov/pubmed/16723430 http://dx.doi.org/10.1093/nar/gkl355 |
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author | Seitz, Erica M. Kowalczykowski, Stephen C. |
author_facet | Seitz, Erica M. Kowalczykowski, Stephen C. |
author_sort | Seitz, Erica M. |
collection | PubMed |
description | DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases. |
format | Text |
id | pubmed-1474073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-14740732006-06-08 Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci Seitz, Erica M. Kowalczykowski, Stephen C. Nucleic Acids Res Article DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases. Oxford University Press 2006 2006-05-24 /pmc/articles/PMC1474073/ /pubmed/16723430 http://dx.doi.org/10.1093/nar/gkl355 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Seitz, Erica M. Kowalczykowski, Stephen C. Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title | Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title_full | Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title_fullStr | Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title_full_unstemmed | Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title_short | Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci |
title_sort | human rad51 protein displays enhanced homologous pairing of dna sequences resembling those at genetically unstable loci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474073/ https://www.ncbi.nlm.nih.gov/pubmed/16723430 http://dx.doi.org/10.1093/nar/gkl355 |
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