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Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci

DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapi...

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Autores principales: Seitz, Erica M., Kowalczykowski, Stephen C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474073/
https://www.ncbi.nlm.nih.gov/pubmed/16723430
http://dx.doi.org/10.1093/nar/gkl355
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author Seitz, Erica M.
Kowalczykowski, Stephen C.
author_facet Seitz, Erica M.
Kowalczykowski, Stephen C.
author_sort Seitz, Erica M.
collection PubMed
description DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.
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spelling pubmed-14740732006-06-08 Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci Seitz, Erica M. Kowalczykowski, Stephen C. Nucleic Acids Res Article DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases. Oxford University Press 2006 2006-05-24 /pmc/articles/PMC1474073/ /pubmed/16723430 http://dx.doi.org/10.1093/nar/gkl355 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Seitz, Erica M.
Kowalczykowski, Stephen C.
Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title_full Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title_fullStr Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title_full_unstemmed Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title_short Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci
title_sort human rad51 protein displays enhanced homologous pairing of dna sequences resembling those at genetically unstable loci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474073/
https://www.ncbi.nlm.nih.gov/pubmed/16723430
http://dx.doi.org/10.1093/nar/gkl355
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