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Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus.
A low molecular weight Cd-binding phosphoglycoprotein, cadmium-mycophosphatin, has been isolated from the mushroom Agaricus macrosporus. This protein has a molecular weight of 12,000 dalton and contains no sulfur but a high amount of acid amino acids (Glu, Asp), and carbohydrates (glucose, galactose...
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Formato: | Texto |
Lenguaje: | English |
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1986
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474717/ https://www.ncbi.nlm.nih.gov/pubmed/3709455 |
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author | Meisch, H U Schmitt, J A |
author_facet | Meisch, H U Schmitt, J A |
author_sort | Meisch, H U |
collection | PubMed |
description | A low molecular weight Cd-binding phosphoglycoprotein, cadmium-mycophosphatin, has been isolated from the mushroom Agaricus macrosporus. This protein has a molecular weight of 12,000 dalton and contains no sulfur but a high amount of acid amino acids (Glu, Asp), and carbohydrates (glucose, galactose). Cadmium-mycophosphatin has an isoelectric point less than pH 2, binds cadmium with a dissociation constant of KD = 1.59 X 10 M (pKD = 6.8) and is saturated with 13.5 mole Cd/mole, all Cd-binding sites being equivalent. It is suggested that Cd is bound by phosphoserine groups, similar relations being known from calcium-binding proteins in animals. From A. macrosporus four other low-molecular weight glycoproteins have been isolated which contain sulfur and bind cadmium and copper. The biological significance of these Cd-binding proteins is discussed. |
format | Text |
id | pubmed-1474717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1986 |
record_format | MEDLINE/PubMed |
spelling | pubmed-14747172006-06-09 Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. Meisch, H U Schmitt, J A Environ Health Perspect Research Article A low molecular weight Cd-binding phosphoglycoprotein, cadmium-mycophosphatin, has been isolated from the mushroom Agaricus macrosporus. This protein has a molecular weight of 12,000 dalton and contains no sulfur but a high amount of acid amino acids (Glu, Asp), and carbohydrates (glucose, galactose). Cadmium-mycophosphatin has an isoelectric point less than pH 2, binds cadmium with a dissociation constant of KD = 1.59 X 10 M (pKD = 6.8) and is saturated with 13.5 mole Cd/mole, all Cd-binding sites being equivalent. It is suggested that Cd is bound by phosphoserine groups, similar relations being known from calcium-binding proteins in animals. From A. macrosporus four other low-molecular weight glycoproteins have been isolated which contain sulfur and bind cadmium and copper. The biological significance of these Cd-binding proteins is discussed. 1986-03 /pmc/articles/PMC1474717/ /pubmed/3709455 Text en |
spellingShingle | Research Article Meisch, H U Schmitt, J A Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title | Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title_full | Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title_fullStr | Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title_full_unstemmed | Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title_short | Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus. |
title_sort | characterization studies on cadmium-mycophosphatin from the mushroom agaricus macrosporus. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474717/ https://www.ncbi.nlm.nih.gov/pubmed/3709455 |
work_keys_str_mv | AT meischhu characterizationstudiesoncadmiummycophosphatinfromthemushroomagaricusmacrosporus AT schmittja characterizationstudiesoncadmiummycophosphatinfromthemushroomagaricusmacrosporus |