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Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.

The occurrence of high cadmium levels in the digestive gland (hepatopancreas) of the crab Cancer pagurus is reviewed and its possible significance to human health is discussed. Previous attempts to isolate this cadmium in its native form are briefly reviewed and the present method used in this labor...

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Detalles Bibliográficos
Autor principal: Overnell, J
Formato: Texto
Lenguaje:English
Publicado: 1986
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474718/
https://www.ncbi.nlm.nih.gov/pubmed/3709430
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author Overnell, J
author_facet Overnell, J
author_sort Overnell, J
collection PubMed
description The occurrence of high cadmium levels in the digestive gland (hepatopancreas) of the crab Cancer pagurus is reviewed and its possible significance to human health is discussed. Previous attempts to isolate this cadmium in its native form are briefly reviewed and the present method used in this laboratory to isolate crab cadmium metallothionein is described. Quantitative amino acid analysis of the purified protein suggests that it has a total of 55 residues, including 19 Cys, and a molecular weight of approximately 5800. This yields an extinction coefficient at 220 nm for the protein in HCl, pH 1.5, of 50,000 and at 250 nm for the native cadmium protein in Tris-HCl, pH 8.4, of 92,000.
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spelling pubmed-14747182006-06-09 Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein. Overnell, J Environ Health Perspect Research Article The occurrence of high cadmium levels in the digestive gland (hepatopancreas) of the crab Cancer pagurus is reviewed and its possible significance to human health is discussed. Previous attempts to isolate this cadmium in its native form are briefly reviewed and the present method used in this laboratory to isolate crab cadmium metallothionein is described. Quantitative amino acid analysis of the purified protein suggests that it has a total of 55 residues, including 19 Cys, and a molecular weight of approximately 5800. This yields an extinction coefficient at 220 nm for the protein in HCl, pH 1.5, of 50,000 and at 250 nm for the native cadmium protein in Tris-HCl, pH 8.4, of 92,000. 1986-03 /pmc/articles/PMC1474718/ /pubmed/3709430 Text en
spellingShingle Research Article
Overnell, J
Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title_full Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title_fullStr Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title_full_unstemmed Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title_short Occurrence of cadmium in crabs (Cancer pagurus) and the isolation and properties of cadmium metallothionein.
title_sort occurrence of cadmium in crabs (cancer pagurus) and the isolation and properties of cadmium metallothionein.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474718/
https://www.ncbi.nlm.nih.gov/pubmed/3709430
work_keys_str_mv AT overnellj occurrenceofcadmiumincrabscancerpagurusandtheisolationandpropertiesofcadmiummetallothionein