Fast, easy and efficient: site-specific insertion of transgenes into Enterobacterial chromosomes using Tn7 without need for selection of the insertion event

BACKGROUND: Inserting transgenes into bacterial chromosomes is generally quite involved, requiring a selection for cells carrying the insertion, usually for drug-resistance, or multiple cumbersome manipulations, or both. Several approaches use phage λ red recombination, which allows for the possibil...

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Detalles Bibliográficos
Autores principales: McKenzie, Gregory J, Craig, Nancy L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475584/
https://www.ncbi.nlm.nih.gov/pubmed/16646962
http://dx.doi.org/10.1186/1471-2180-6-39

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475584/
https://www.ncbi.nlm.nih.gov/pubmed/16646962
http://dx.doi.org/10.1186/1471-2180-6-39

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