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Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis
BACKGROUND: Despite bacteraemia is present in the majority of patients with pneumococcal, little is known about the influence of the systemic infection on the meningeal inflammatory response. METHODS: To explore the role of systemic infection on the meningeal inflammation, experimental meningitis wa...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475592/ https://www.ncbi.nlm.nih.gov/pubmed/16643642 http://dx.doi.org/10.1186/1471-2334-6-78 |
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author | ∅stergaard, Christian O'Reilly, Terence Brandt, Christian Frimodt-Møller, Niels Lundgren, Jens D |
author_facet | ∅stergaard, Christian O'Reilly, Terence Brandt, Christian Frimodt-Møller, Niels Lundgren, Jens D |
author_sort | ∅stergaard, Christian |
collection | PubMed |
description | BACKGROUND: Despite bacteraemia is present in the majority of patients with pneumococcal, little is known about the influence of the systemic infection on the meningeal inflammatory response. METHODS: To explore the role of systemic infection on the meningeal inflammation, experimental meningitis was induced by intracisternal injection of ~1 × 10(6 )CFU Streptococcus pneumoniae, type 3, and the 26 rabbits were either provided with ~1 × 10(6 )CFU S. pneumoniae intravenously at 0 hour ("bacteraemic" rabbits, n = 9), immunized with paraformaldehyde-killed S. pneumoniae for 5 weeks prior to the experiment ("immunized" rabbits", n = 8), or not treated further ("control" rabbits, n = 9). WBC and bacterial concentrations were determined in CSF and blood every second hour during a 16 hours study period together with CSF IL-8 and protein levels. We also studied CSF and blood WBC levels in 153 pneumococcal meningitis patients with and without presence of bacteraemia. RESULTS: As designed, blood bacterial concentrations were significantly different among three experimental groups during the 16 hours study period (Kruskal Wallis test, P < 0.05), whereas no differences in CSF bacterial levels were observed (P > 0.05). Blood WBC decreased in bacteraemic rabbits between ~10–16 hours after the bacterial inoculation in contrast to an increase for both the immunized rabbits and controls (P < 0.05). The CSF pleocytosis was attenuated in bacteraemic rabbits as compared to the two other groups between 12–16 hours from time of infection (P < 0.017), despite accelerated CSF IL-8 levels in bacteraemic rabbits. In patients with pneumococcal meningitis, no significant difference in CSF WBC was observed between patients with or without bacteraemia at admission (n = 103, 1740 cells/μL (123–4032) vs. n = 50, 1961 cells/μL (673–5182), respectively, P = 0.18), but there was a significant correlation between CSF and blood WBC (n = 127, Spearman rho = 0.234, P = 0.008). CONCLUSION: Our results suggest that a decrease in peripheral WBC induced by enhanced bacteraemia in pneumococcal meningitis results in an attenuated CSF pleocytosis. |
format | Text |
id | pubmed-1475592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-14755922006-06-08 Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis ∅stergaard, Christian O'Reilly, Terence Brandt, Christian Frimodt-Møller, Niels Lundgren, Jens D BMC Infect Dis Research Article BACKGROUND: Despite bacteraemia is present in the majority of patients with pneumococcal, little is known about the influence of the systemic infection on the meningeal inflammatory response. METHODS: To explore the role of systemic infection on the meningeal inflammation, experimental meningitis was induced by intracisternal injection of ~1 × 10(6 )CFU Streptococcus pneumoniae, type 3, and the 26 rabbits were either provided with ~1 × 10(6 )CFU S. pneumoniae intravenously at 0 hour ("bacteraemic" rabbits, n = 9), immunized with paraformaldehyde-killed S. pneumoniae for 5 weeks prior to the experiment ("immunized" rabbits", n = 8), or not treated further ("control" rabbits, n = 9). WBC and bacterial concentrations were determined in CSF and blood every second hour during a 16 hours study period together with CSF IL-8 and protein levels. We also studied CSF and blood WBC levels in 153 pneumococcal meningitis patients with and without presence of bacteraemia. RESULTS: As designed, blood bacterial concentrations were significantly different among three experimental groups during the 16 hours study period (Kruskal Wallis test, P < 0.05), whereas no differences in CSF bacterial levels were observed (P > 0.05). Blood WBC decreased in bacteraemic rabbits between ~10–16 hours after the bacterial inoculation in contrast to an increase for both the immunized rabbits and controls (P < 0.05). The CSF pleocytosis was attenuated in bacteraemic rabbits as compared to the two other groups between 12–16 hours from time of infection (P < 0.017), despite accelerated CSF IL-8 levels in bacteraemic rabbits. In patients with pneumococcal meningitis, no significant difference in CSF WBC was observed between patients with or without bacteraemia at admission (n = 103, 1740 cells/μL (123–4032) vs. n = 50, 1961 cells/μL (673–5182), respectively, P = 0.18), but there was a significant correlation between CSF and blood WBC (n = 127, Spearman rho = 0.234, P = 0.008). CONCLUSION: Our results suggest that a decrease in peripheral WBC induced by enhanced bacteraemia in pneumococcal meningitis results in an attenuated CSF pleocytosis. BioMed Central 2006-04-27 /pmc/articles/PMC1475592/ /pubmed/16643642 http://dx.doi.org/10.1186/1471-2334-6-78 Text en Copyright © 2006 ∅stergaard et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article ∅stergaard, Christian O'Reilly, Terence Brandt, Christian Frimodt-Møller, Niels Lundgren, Jens D Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title | Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title_full | Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title_fullStr | Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title_full_unstemmed | Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title_short | Influence of the blood bacterial load on the meningeal inflammatory response in Streptococcus pneumoniae meningitis |
title_sort | influence of the blood bacterial load on the meningeal inflammatory response in streptococcus pneumoniae meningitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475592/ https://www.ncbi.nlm.nih.gov/pubmed/16643642 http://dx.doi.org/10.1186/1471-2334-6-78 |
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