Localization of deformed wing virus infection in queen and drone Apis mellifera L

The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' porti...

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Autores principales: Fievet, Julie, Tentcheva, Diana, Gauthier, Laurent, de Miranda, Joachim, Cousserans, François, Colin, Marc Edouard, Bergoin, Max
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475838/
https://www.ncbi.nlm.nih.gov/pubmed/16569216
http://dx.doi.org/10.1186/1743-422X-3-16
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author Fievet, Julie
Tentcheva, Diana
Gauthier, Laurent
de Miranda, Joachim
Cousserans, François
Colin, Marc Edouard
Bergoin, Max
author_facet Fievet, Julie
Tentcheva, Diana
Gauthier, Laurent
de Miranda, Joachim
Cousserans, François
Colin, Marc Edouard
Bergoin, Max
author_sort Fievet, Julie
collection PubMed
description The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VP1 structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles.
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spelling pubmed-14758382006-06-10 Localization of deformed wing virus infection in queen and drone Apis mellifera L Fievet, Julie Tentcheva, Diana Gauthier, Laurent de Miranda, Joachim Cousserans, François Colin, Marc Edouard Bergoin, Max Virol J Short Report The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VP1 structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles. BioMed Central 2006-03-28 /pmc/articles/PMC1475838/ /pubmed/16569216 http://dx.doi.org/10.1186/1743-422X-3-16 Text en Copyright © 2006 Fievet et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Fievet, Julie
Tentcheva, Diana
Gauthier, Laurent
de Miranda, Joachim
Cousserans, François
Colin, Marc Edouard
Bergoin, Max
Localization of deformed wing virus infection in queen and drone Apis mellifera L
title Localization of deformed wing virus infection in queen and drone Apis mellifera L
title_full Localization of deformed wing virus infection in queen and drone Apis mellifera L
title_fullStr Localization of deformed wing virus infection in queen and drone Apis mellifera L
title_full_unstemmed Localization of deformed wing virus infection in queen and drone Apis mellifera L
title_short Localization of deformed wing virus infection in queen and drone Apis mellifera L
title_sort localization of deformed wing virus infection in queen and drone apis mellifera l
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475838/
https://www.ncbi.nlm.nih.gov/pubmed/16569216
http://dx.doi.org/10.1186/1743-422X-3-16
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