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Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis
BACKGROUND: In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito Anopheles arabiensis Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined. METHOD...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475870/ https://www.ncbi.nlm.nih.gov/pubmed/16700906 http://dx.doi.org/10.1186/1475-2875-5-41 |
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author | Helinski, Michelle EH Parker, Andrew G Knols, Bart GJ |
author_facet | Helinski, Michelle EH Parker, Andrew G Knols, Bart GJ |
author_sort | Helinski, Michelle EH |
collection | PubMed |
description | BACKGROUND: In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito Anopheles arabiensis Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined. METHODS: Pupae were irradiated shortly before emergence (at 22–26 hrs of age), and adults <24 hrs post emergence. Doses tested ranged between 0 and 100 Gy. The effects of irradiation on adult emergence, male survival, induced sterility and insemination capability were evaluated. Emergence and insemination data were analysed using independent t-tests against the control. Correlation analyses were performed for insemination rate and dose and insemination and fecundity. Male survival was analysed using Kaplan-Meier survival analyses. Finally, the calculated residual fertility values were inverse-normal transformed and linear regression analyses performed. RESULTS: Irradiation of pupae, for all doses tested, had no effect on adult emergence. Survival curves of males irradiated as pupae or adults were similar or even slightly higher than non-irradiated males. Overall, adults appeared to be slightly more susceptible to irradiation, although no significant differences for individual doses were observed. In the pupal stage, a significant negative correlation was found between insemination and dose, but the correlation-coefficient was associated with less than 25% of the total variation. A review of the literature indicated that An. arabiensis is more radiation resistant than other anopheline mosquitoes. CONCLUSION: The optimal dose for male insects to be released in an SIT programme depends on their level of sterility and competitiveness. The use of semi-sterilizing doses to produce more competitive insects is discussed. The most convenient developmental stage for mosquito irradiation on a mass-scale are pupae, but pupal irradiation resulted in a lower insemination rate at the highest dose compared to adult irradiation. On the basis of this study, a suitable dose range that includes semi-sterilizing doses is identified to initiate competitiveness experiments for males irradiated at both developmental stages. |
format | Text |
id | pubmed-1475870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-14758702006-06-10 Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis Helinski, Michelle EH Parker, Andrew G Knols, Bart GJ Malar J Research BACKGROUND: In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito Anopheles arabiensis Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined. METHODS: Pupae were irradiated shortly before emergence (at 22–26 hrs of age), and adults <24 hrs post emergence. Doses tested ranged between 0 and 100 Gy. The effects of irradiation on adult emergence, male survival, induced sterility and insemination capability were evaluated. Emergence and insemination data were analysed using independent t-tests against the control. Correlation analyses were performed for insemination rate and dose and insemination and fecundity. Male survival was analysed using Kaplan-Meier survival analyses. Finally, the calculated residual fertility values were inverse-normal transformed and linear regression analyses performed. RESULTS: Irradiation of pupae, for all doses tested, had no effect on adult emergence. Survival curves of males irradiated as pupae or adults were similar or even slightly higher than non-irradiated males. Overall, adults appeared to be slightly more susceptible to irradiation, although no significant differences for individual doses were observed. In the pupal stage, a significant negative correlation was found between insemination and dose, but the correlation-coefficient was associated with less than 25% of the total variation. A review of the literature indicated that An. arabiensis is more radiation resistant than other anopheline mosquitoes. CONCLUSION: The optimal dose for male insects to be released in an SIT programme depends on their level of sterility and competitiveness. The use of semi-sterilizing doses to produce more competitive insects is discussed. The most convenient developmental stage for mosquito irradiation on a mass-scale are pupae, but pupal irradiation resulted in a lower insemination rate at the highest dose compared to adult irradiation. On the basis of this study, a suitable dose range that includes semi-sterilizing doses is identified to initiate competitiveness experiments for males irradiated at both developmental stages. BioMed Central 2006-05-15 /pmc/articles/PMC1475870/ /pubmed/16700906 http://dx.doi.org/10.1186/1475-2875-5-41 Text en Copyright © 2006 Helinski et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Helinski, Michelle EH Parker, Andrew G Knols, Bart GJ Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title | Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title_full | Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title_fullStr | Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title_full_unstemmed | Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title_short | Radiation-induced sterility for pupal and adult stages of the malaria mosquito Anopheles arabiensis |
title_sort | radiation-induced sterility for pupal and adult stages of the malaria mosquito anopheles arabiensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475870/ https://www.ncbi.nlm.nih.gov/pubmed/16700906 http://dx.doi.org/10.1186/1475-2875-5-41 |
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