Cargando…

Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya

BACKGROUND: The successful development of odour-baited trapping systems for mosquitoes depends on the identification of behaviourally active semiochemicals, besides the design and operating principles of such devices. A large variety of 'attractants' has been identified in laboratory inves...

Descripción completa

Detalles Bibliográficos
Autores principales: Njiru, Basilio N, Mukabana, Wolfgang R, Takken, Willem, Knols, Bart GJ
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475871/
https://www.ncbi.nlm.nih.gov/pubmed/16700902
http://dx.doi.org/10.1186/1475-2875-5-39
_version_ 1782128161590345728
author Njiru, Basilio N
Mukabana, Wolfgang R
Takken, Willem
Knols, Bart GJ
author_facet Njiru, Basilio N
Mukabana, Wolfgang R
Takken, Willem
Knols, Bart GJ
author_sort Njiru, Basilio N
collection PubMed
description BACKGROUND: The successful development of odour-baited trapping systems for mosquitoes depends on the identification of behaviourally active semiochemicals, besides the design and operating principles of such devices. A large variety of 'attractants' has been identified in laboratory investigations, yet few of these increase trap catches in the field. A contained system, intermediate between the laboratory and open field, is presented and previous reports that human foot odour induces behavioural responses of Anopheles gambiae confirmed. METHODS: The response of 3–5 day old female An. gambiae towards odour-baited counterflow geometry traps (MM-X model; American Biophysics Corp., RI) was studied in semi-field (screen house) conditions in western Kenya. Traps were baited with human foot odour (collected on socks), carbon dioxide (CO(2), 500 ml min(-1)), ammonia (NH(3)), 1-octen-3-ol, or various combinations thereof. Trap catches were log (x+1) transformed and subjected to Latin square analysis of variance procedures. RESULTS: Apart from 1-octen-3-ol, all odour baits caused significant (P < 0.05) increases in trap catches over non-baited traps. Foot odour remained behaviourally active for at least 8 days after collection on nylon or cotton sock fabric. A synergistic response (P < 0.001) was observed towards the combination of foot odour and CO(2), which increased catches of these odours alone by 3.8 and 2.7 times, respectively. CONCLUSION: These results are the first to report behavioural responses of an African malaria vector to human foot odour outside the laboratory, and further investigation of fractions and/or individual chemical components of this odour complex are called for. Semi-field systems offer the prospect of high-throughput screening of candidate kairomones, which may expedite the development of efficient trap-bait systems for this and other African mosquito species.
format Text
id pubmed-1475871
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-14758712006-06-10 Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya Njiru, Basilio N Mukabana, Wolfgang R Takken, Willem Knols, Bart GJ Malar J Research BACKGROUND: The successful development of odour-baited trapping systems for mosquitoes depends on the identification of behaviourally active semiochemicals, besides the design and operating principles of such devices. A large variety of 'attractants' has been identified in laboratory investigations, yet few of these increase trap catches in the field. A contained system, intermediate between the laboratory and open field, is presented and previous reports that human foot odour induces behavioural responses of Anopheles gambiae confirmed. METHODS: The response of 3–5 day old female An. gambiae towards odour-baited counterflow geometry traps (MM-X model; American Biophysics Corp., RI) was studied in semi-field (screen house) conditions in western Kenya. Traps were baited with human foot odour (collected on socks), carbon dioxide (CO(2), 500 ml min(-1)), ammonia (NH(3)), 1-octen-3-ol, or various combinations thereof. Trap catches were log (x+1) transformed and subjected to Latin square analysis of variance procedures. RESULTS: Apart from 1-octen-3-ol, all odour baits caused significant (P < 0.05) increases in trap catches over non-baited traps. Foot odour remained behaviourally active for at least 8 days after collection on nylon or cotton sock fabric. A synergistic response (P < 0.001) was observed towards the combination of foot odour and CO(2), which increased catches of these odours alone by 3.8 and 2.7 times, respectively. CONCLUSION: These results are the first to report behavioural responses of an African malaria vector to human foot odour outside the laboratory, and further investigation of fractions and/or individual chemical components of this odour complex are called for. Semi-field systems offer the prospect of high-throughput screening of candidate kairomones, which may expedite the development of efficient trap-bait systems for this and other African mosquito species. BioMed Central 2006-05-15 /pmc/articles/PMC1475871/ /pubmed/16700902 http://dx.doi.org/10.1186/1475-2875-5-39 Text en Copyright © 2006 Njiru et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Njiru, Basilio N
Mukabana, Wolfgang R
Takken, Willem
Knols, Bart GJ
Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title_full Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title_fullStr Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title_full_unstemmed Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title_short Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
title_sort trapping of the malaria vector anopheles gambiae with odour-baited mm-x traps in semi-field conditions in western kenya
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1475871/
https://www.ncbi.nlm.nih.gov/pubmed/16700902
http://dx.doi.org/10.1186/1475-2875-5-39
work_keys_str_mv AT njirubasilion trappingofthemalariavectoranophelesgambiaewithodourbaitedmmxtrapsinsemifieldconditionsinwesternkenya
AT mukabanawolfgangr trappingofthemalariavectoranophelesgambiaewithodourbaitedmmxtrapsinsemifieldconditionsinwesternkenya
AT takkenwillem trappingofthemalariavectoranophelesgambiaewithodourbaitedmmxtrapsinsemifieldconditionsinwesternkenya
AT knolsbartgj trappingofthemalariavectoranophelesgambiaewithodourbaitedmmxtrapsinsemifieldconditionsinwesternkenya