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Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates

Four primate (PTLV), human (HTLV) and simian (STLV) T-cell leukemia virus types, have been characterized thus far, with evidence of a simian zoonotic origin for HTLV-1, HTLV-2 and HTLV-3 in Africa. The PTLV envelope glycoprotein surface component (SUgp46) comprises a receptor-binding domain (RBD) th...

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Autores principales: Kim, Felix J, Lavanya, Madakasira, Gessain, Antoine, Gallego, Sandra, Battini, Jean-Luc, Sitbon, Marc, Courgnaud, Valérie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481548/
https://www.ncbi.nlm.nih.gov/pubmed/16725042
http://dx.doi.org/10.1186/1742-4690-3-29
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author Kim, Felix J
Lavanya, Madakasira
Gessain, Antoine
Gallego, Sandra
Battini, Jean-Luc
Sitbon, Marc
Courgnaud, Valérie
author_facet Kim, Felix J
Lavanya, Madakasira
Gessain, Antoine
Gallego, Sandra
Battini, Jean-Luc
Sitbon, Marc
Courgnaud, Valérie
author_sort Kim, Felix J
collection PubMed
description Four primate (PTLV), human (HTLV) and simian (STLV) T-cell leukemia virus types, have been characterized thus far, with evidence of a simian zoonotic origin for HTLV-1, HTLV-2 and HTLV-3 in Africa. The PTLV envelope glycoprotein surface component (SUgp46) comprises a receptor-binding domain (RBD) that alternates hypervariable and highly conserved sequences. To further delineate highly conserved motifs in PTLV RBDs, we investigated the intrahost variability of HTLV-1 and STLV-1 by generating and sequencing libraries of DNA fragments amplified within the RBD of the SUgp46 env gene. Using new and highly cross-reactive env primer pairs, we observed the presence of Env quasispecies in HTLV-1 infected individuals and STLV-1 naturally infected macaques, irrespective of the clinical status. These intrahost variants helped us to define highly conserved residues and motifs in the RBD. The new highly sensitive env PCR described here appears suitable for the screening of all known variants of the different PTLV types and should, therefore, be useful for the analysis of seroindeterminate samples.
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spelling pubmed-14815482006-06-22 Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates Kim, Felix J Lavanya, Madakasira Gessain, Antoine Gallego, Sandra Battini, Jean-Luc Sitbon, Marc Courgnaud, Valérie Retrovirology Short Report Four primate (PTLV), human (HTLV) and simian (STLV) T-cell leukemia virus types, have been characterized thus far, with evidence of a simian zoonotic origin for HTLV-1, HTLV-2 and HTLV-3 in Africa. The PTLV envelope glycoprotein surface component (SUgp46) comprises a receptor-binding domain (RBD) that alternates hypervariable and highly conserved sequences. To further delineate highly conserved motifs in PTLV RBDs, we investigated the intrahost variability of HTLV-1 and STLV-1 by generating and sequencing libraries of DNA fragments amplified within the RBD of the SUgp46 env gene. Using new and highly cross-reactive env primer pairs, we observed the presence of Env quasispecies in HTLV-1 infected individuals and STLV-1 naturally infected macaques, irrespective of the clinical status. These intrahost variants helped us to define highly conserved residues and motifs in the RBD. The new highly sensitive env PCR described here appears suitable for the screening of all known variants of the different PTLV types and should, therefore, be useful for the analysis of seroindeterminate samples. BioMed Central 2006-05-25 /pmc/articles/PMC1481548/ /pubmed/16725042 http://dx.doi.org/10.1186/1742-4690-3-29 Text en Copyright © 2006 Kim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Kim, Felix J
Lavanya, Madakasira
Gessain, Antoine
Gallego, Sandra
Battini, Jean-Luc
Sitbon, Marc
Courgnaud, Valérie
Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title_full Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title_fullStr Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title_full_unstemmed Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title_short Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates
title_sort intrahost variations in the envelope receptor-binding domain (rbd) of htlv-1 and stlv-1 primary isolates
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481548/
https://www.ncbi.nlm.nih.gov/pubmed/16725042
http://dx.doi.org/10.1186/1742-4690-3-29
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