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Coexistence of K-ras mutations and HPV infection in colon cancer
BACKGROUND: Activation of the ras genes or association with human papillomavirus infection have been extensively studied in colorectal cancer. However, the correlation between K-ras mutations and HPV in colorectal cancer has not been investigated yet. In this study we aimed to investigate the presen...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481576/ https://www.ncbi.nlm.nih.gov/pubmed/16672071 http://dx.doi.org/10.1186/1471-2407-6-115 |
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author | Buyru, Nur Tezol, Ayda Dalay, Nejat |
author_facet | Buyru, Nur Tezol, Ayda Dalay, Nejat |
author_sort | Buyru, Nur |
collection | PubMed |
description | BACKGROUND: Activation of the ras genes or association with human papillomavirus infection have been extensively studied in colorectal cancer. However, the correlation between K-ras mutations and HPV in colorectal cancer has not been investigated yet. In this study we aimed to investigate the presence of K-ras mutations and their correlation with HPV infection in colon cancer. METHODS: K-ras mutations were analyzed by a mutagenic PCR assay and digestion with specific restriction enzymes to distinguish the wild-type and mutant codons. HPV infection was analyzed by PCR amplification and hybridization with specific probes by Southern blotting. Stattistical analyses were performed by the chi-square and Fisher's exact tests RESULTS: HPV gene fragments were detected in 43 tumors and 17 normal tissue samples. HPV 18 was the prevalent type in the tumor tissue. A mutation at codon 12 of the K-ras gene was present in 31 patients. 56% of the HPV-positive tumors also harbored a K-ras mutation. Codon 13 mutations were not observed. These data indicate that infection with high risk HPV types and mutational activation of the K-ras gene are frequent events in colorectal carcinogenesis. CONCLUSION: Our findings suggest that mutational activation of the K-ras gene is a common event in colon carcinogenesis and that HPV infection may represent an important factor in the development of the premalignant lesions leading to the neoplastic phenotype. |
format | Text |
id | pubmed-1481576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-14815762006-06-22 Coexistence of K-ras mutations and HPV infection in colon cancer Buyru, Nur Tezol, Ayda Dalay, Nejat BMC Cancer Research Article BACKGROUND: Activation of the ras genes or association with human papillomavirus infection have been extensively studied in colorectal cancer. However, the correlation between K-ras mutations and HPV in colorectal cancer has not been investigated yet. In this study we aimed to investigate the presence of K-ras mutations and their correlation with HPV infection in colon cancer. METHODS: K-ras mutations were analyzed by a mutagenic PCR assay and digestion with specific restriction enzymes to distinguish the wild-type and mutant codons. HPV infection was analyzed by PCR amplification and hybridization with specific probes by Southern blotting. Stattistical analyses were performed by the chi-square and Fisher's exact tests RESULTS: HPV gene fragments were detected in 43 tumors and 17 normal tissue samples. HPV 18 was the prevalent type in the tumor tissue. A mutation at codon 12 of the K-ras gene was present in 31 patients. 56% of the HPV-positive tumors also harbored a K-ras mutation. Codon 13 mutations were not observed. These data indicate that infection with high risk HPV types and mutational activation of the K-ras gene are frequent events in colorectal carcinogenesis. CONCLUSION: Our findings suggest that mutational activation of the K-ras gene is a common event in colon carcinogenesis and that HPV infection may represent an important factor in the development of the premalignant lesions leading to the neoplastic phenotype. BioMed Central 2006-05-04 /pmc/articles/PMC1481576/ /pubmed/16672071 http://dx.doi.org/10.1186/1471-2407-6-115 Text en Copyright © 2006 Buyru et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Buyru, Nur Tezol, Ayda Dalay, Nejat Coexistence of K-ras mutations and HPV infection in colon cancer |
title | Coexistence of K-ras mutations and HPV infection in colon cancer |
title_full | Coexistence of K-ras mutations and HPV infection in colon cancer |
title_fullStr | Coexistence of K-ras mutations and HPV infection in colon cancer |
title_full_unstemmed | Coexistence of K-ras mutations and HPV infection in colon cancer |
title_short | Coexistence of K-ras mutations and HPV infection in colon cancer |
title_sort | coexistence of k-ras mutations and hpv infection in colon cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481576/ https://www.ncbi.nlm.nih.gov/pubmed/16672071 http://dx.doi.org/10.1186/1471-2407-6-115 |
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