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Primer Extension Enrichment Reaction (PEER): a new subtraction method for identification of genetic differences between biological specimens

We developed a conceptually new subtraction strategy for the detection and isolation of target DNA and/or RNA from complex nucleic acid mixtures, called Primer Extension Enrichment Reaction (PEER). PEER uses adapters and class IIS restriction enzymes to generate tagged oligonucleotides from dsDNA fr...

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Detalles Bibliográficos
Autores principales: Ganova-Raeva, Lilia, Zhang, Xinjian, Cao, Fengli, Fields, Howard, Khudyakov, Yury
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1484250/
https://www.ncbi.nlm.nih.gov/pubmed/16790564
http://dx.doi.org/10.1093/nar/gkl391