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Primer Extension Enrichment Reaction (PEER): a new subtraction method for identification of genetic differences between biological specimens
We developed a conceptually new subtraction strategy for the detection and isolation of target DNA and/or RNA from complex nucleic acid mixtures, called Primer Extension Enrichment Reaction (PEER). PEER uses adapters and class IIS restriction enzymes to generate tagged oligonucleotides from dsDNA fr...
Autores principales: | Ganova-Raeva, Lilia, Zhang, Xinjian, Cao, Fengli, Fields, Howard, Khudyakov, Yury |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1484250/ https://www.ncbi.nlm.nih.gov/pubmed/16790564 http://dx.doi.org/10.1093/nar/gkl391 |
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