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Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing

BACKGROUND: Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restrict...

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Autores principales: Arricau-Bouvery, Nathalie, Hauck, Yolande, Bejaoui, Awatef, Frangoulidis, Dimitrios, Bodier, Christelle C, Souriau, Armel, Meyer, Hermann, Neubauer, Heinrich, Rodolakis, Annie, Vergnaud, Gilles
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1488860/
https://www.ncbi.nlm.nih.gov/pubmed/16640773
http://dx.doi.org/10.1186/1471-2180-6-38
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author Arricau-Bouvery, Nathalie
Hauck, Yolande
Bejaoui, Awatef
Frangoulidis, Dimitrios
Bodier, Christelle C
Souriau, Armel
Meyer, Hermann
Neubauer, Heinrich
Rodolakis, Annie
Vergnaud, Gilles
author_facet Arricau-Bouvery, Nathalie
Hauck, Yolande
Bejaoui, Awatef
Frangoulidis, Dimitrios
Bodier, Christelle C
Souriau, Armel
Meyer, Hermann
Neubauer, Heinrich
Rodolakis, Annie
Vergnaud, Gilles
author_sort Arricau-Bouvery, Nathalie
collection PubMed
description BACKGROUND: Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restriction site-PCR (IRS-PCR) and multiple loci variable number of tandem repeats (VNTR) analysis (MLVA). RESULTS: By applying IRS-PCR, 14 C. burnetii isolates could be divided into six groups containing up to five different isolates. Clustering as deduced from MLVA typing with 17 markers provided an increased resolution with an excellent agreement to IRS-PCR, and with the plasmid type of each strain. MLVA was then applied to 28 additional C. burnetii isolates of different origin and 36 different genotypes were identified among the 42 isolates investigated. The clustering obtained is in agreement with published Multiple Locus Sequence Typing (MLST) data. Two panels of markers are proposed, panel 1 which can be confidently typed on agarose gel at a lower cost and in any laboratory setting (10 minisatellite markers with a repeat unit larger than 9 bp), and panel 2 which comprises 7 microsatellites and provides a higher discriminatory power. CONCLUSION: Our analyses demonstrate that MLVA is a powerful and promising molecular typing tool with a high resolution and of low costs. The consistency of the results with independent methods suggests that MLVA can be applied for epidemiological studies. The resulting data can be queried on a dedicated MLVA genotyping Web service.
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spelling pubmed-14888602006-07-06 Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing Arricau-Bouvery, Nathalie Hauck, Yolande Bejaoui, Awatef Frangoulidis, Dimitrios Bodier, Christelle C Souriau, Armel Meyer, Hermann Neubauer, Heinrich Rodolakis, Annie Vergnaud, Gilles BMC Microbiol Research Article BACKGROUND: Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restriction site-PCR (IRS-PCR) and multiple loci variable number of tandem repeats (VNTR) analysis (MLVA). RESULTS: By applying IRS-PCR, 14 C. burnetii isolates could be divided into six groups containing up to five different isolates. Clustering as deduced from MLVA typing with 17 markers provided an increased resolution with an excellent agreement to IRS-PCR, and with the plasmid type of each strain. MLVA was then applied to 28 additional C. burnetii isolates of different origin and 36 different genotypes were identified among the 42 isolates investigated. The clustering obtained is in agreement with published Multiple Locus Sequence Typing (MLST) data. Two panels of markers are proposed, panel 1 which can be confidently typed on agarose gel at a lower cost and in any laboratory setting (10 minisatellite markers with a repeat unit larger than 9 bp), and panel 2 which comprises 7 microsatellites and provides a higher discriminatory power. CONCLUSION: Our analyses demonstrate that MLVA is a powerful and promising molecular typing tool with a high resolution and of low costs. The consistency of the results with independent methods suggests that MLVA can be applied for epidemiological studies. The resulting data can be queried on a dedicated MLVA genotyping Web service. BioMed Central 2006-04-26 /pmc/articles/PMC1488860/ /pubmed/16640773 http://dx.doi.org/10.1186/1471-2180-6-38 Text en Copyright © 2006 Arricau-Bouvery et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arricau-Bouvery, Nathalie
Hauck, Yolande
Bejaoui, Awatef
Frangoulidis, Dimitrios
Bodier, Christelle C
Souriau, Armel
Meyer, Hermann
Neubauer, Heinrich
Rodolakis, Annie
Vergnaud, Gilles
Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title_full Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title_fullStr Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title_full_unstemmed Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title_short Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
title_sort molecular characterization of coxiella burnetii isolates by infrequent restriction site-pcr and mlva typing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1488860/
https://www.ncbi.nlm.nih.gov/pubmed/16640773
http://dx.doi.org/10.1186/1471-2180-6-38
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