Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells

BACKGROUND: The phage protein pairs, RecE/RecT from Rac or Redα/Redβ from λ, initiate efficient double strand break repair (DSBR) in Escherichia coli that has proven very useful for DNA engineering. These phage pairs initiate DSBR either by annealing or by another mechanism that is not defined. RESU...

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Detalles Bibliográficos
Autores principales: Zhang, Youming, Muyrers, Joep PP, Rientjes, Jeanette, Stewart, A Francis
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC149363/
https://www.ncbi.nlm.nih.gov/pubmed/12530927
http://dx.doi.org/10.1186/1471-2199-4-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC149363/
https://www.ncbi.nlm.nih.gov/pubmed/12530927
http://dx.doi.org/10.1186/1471-2199-4-1

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