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Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells
BACKGROUND: The phage protein pairs, RecE/RecT from Rac or Redα/Redβ from λ, initiate efficient double strand break repair (DSBR) in Escherichia coli that has proven very useful for DNA engineering. These phage pairs initiate DSBR either by annealing or by another mechanism that is not defined. RESU...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC149363/ https://www.ncbi.nlm.nih.gov/pubmed/12530927 http://dx.doi.org/10.1186/1471-2199-4-1 |