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Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA

BACKGROUND: Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with frozen sections, the staining methods used for histologica...

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Detalles Bibliográficos
Autores principales: Wang, Hongyang, Owens, James D, Shih, Joanna H, Li, Ming-Chung, Bonner, Robert F, Mushinski, J Frederic
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1513394/
https://www.ncbi.nlm.nih.gov/pubmed/16643667
http://dx.doi.org/10.1186/1471-2164-7-97

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