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Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA
BACKGROUND: Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with frozen sections, the staining methods used for histologica...
Autores principales: | Wang, Hongyang, Owens, James D, Shih, Joanna H, Li, Ming-Chung, Bonner, Robert F, Mushinski, J Frederic |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1513394/ https://www.ncbi.nlm.nih.gov/pubmed/16643667 http://dx.doi.org/10.1186/1471-2164-7-97 |
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