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Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish
BACKGROUND: Scrapie and bovine spongiform encephalopathy (BSE) belongs to the group of animal transmissible spongiform encephalopathy (TSE). BSE epidemic in the UK and elsewhere in Europe has been linked to the use of bovine meat and bone meals (MBM) in the feeding of cattle. There is concern that p...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1513558/ https://www.ncbi.nlm.nih.gov/pubmed/16776828 http://dx.doi.org/10.1186/1746-6148-2-21 |
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author | Ingrosso, Loredana Novoa, Beatriz Valle, Andrea Z Dalla Cardone, Franco Aranguren, Raquel Sbriccoli, Marco Bevivino, Simona Iriti, Marcello Liu, Quanguo Vetrugno, Vito Lu, Mei Faoro, Franco Ciappellano, Salvatore Figueras, Antonio Pocchiari, Maurizio |
author_facet | Ingrosso, Loredana Novoa, Beatriz Valle, Andrea Z Dalla Cardone, Franco Aranguren, Raquel Sbriccoli, Marco Bevivino, Simona Iriti, Marcello Liu, Quanguo Vetrugno, Vito Lu, Mei Faoro, Franco Ciappellano, Salvatore Figueras, Antonio Pocchiari, Maurizio |
author_sort | Ingrosso, Loredana |
collection | PubMed |
description | BACKGROUND: Scrapie and bovine spongiform encephalopathy (BSE) belongs to the group of animal transmissible spongiform encephalopathy (TSE). BSE epidemic in the UK and elsewhere in Europe has been linked to the use of bovine meat and bone meals (MBM) in the feeding of cattle. There is concern that pigs, poultry and fish bred for human consumption and fed with infected MBM would eventually develop BSE or carry residual infectivity without disease. Although there has been no evidence of infection in these species, experimental data on the susceptibility to the BSE agent of farm animals other than sheep and cow are limited only to pigs and domestic chicken. In the framework of a EU-granted project we have challenged two species of fish largely used in human food consumption, rainbow trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus), with a mouse-adapted TSE strain (scrapie 139A), to assess the risk related to oral consumption of TSE contaminated food. In trout, we also checked the "in vitro" ability of the pathological isoform of the mouse prion protein (PrP(Sc)) to cross the intestinal epithelium when added to the mucosal side of everted intestine. RESULTS: Fish challenged with a large amount of scrapie mouse brain homogenate by either oral or parenteral routes, showed the ability to clear the majority of infectivity load. None of the fish tissues taken at different time points after oral or parenteral inoculation was able to provoke scrapie disease after intracerebral inoculation in recipient mice. However, a few recipient mice were positive for PrP(Sc )and spongiform lesions in the brain. We also showed a specific binding of PrP(Sc )to the mucosal side of fish intestine in the absence of an active uptake of the prion protein through the intestinal wall. CONCLUSION: These results indicate that scrapie 139A, and possibly BSE, is quickly removed from fish tissues despite evidence of a prion like protein in fish and of a specific binding of PrP(Sc )to the mucosal side of fish intestine. |
format | Text |
id | pubmed-1513558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15135582006-07-22 Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish Ingrosso, Loredana Novoa, Beatriz Valle, Andrea Z Dalla Cardone, Franco Aranguren, Raquel Sbriccoli, Marco Bevivino, Simona Iriti, Marcello Liu, Quanguo Vetrugno, Vito Lu, Mei Faoro, Franco Ciappellano, Salvatore Figueras, Antonio Pocchiari, Maurizio BMC Vet Res Research Article BACKGROUND: Scrapie and bovine spongiform encephalopathy (BSE) belongs to the group of animal transmissible spongiform encephalopathy (TSE). BSE epidemic in the UK and elsewhere in Europe has been linked to the use of bovine meat and bone meals (MBM) in the feeding of cattle. There is concern that pigs, poultry and fish bred for human consumption and fed with infected MBM would eventually develop BSE or carry residual infectivity without disease. Although there has been no evidence of infection in these species, experimental data on the susceptibility to the BSE agent of farm animals other than sheep and cow are limited only to pigs and domestic chicken. In the framework of a EU-granted project we have challenged two species of fish largely used in human food consumption, rainbow trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus), with a mouse-adapted TSE strain (scrapie 139A), to assess the risk related to oral consumption of TSE contaminated food. In trout, we also checked the "in vitro" ability of the pathological isoform of the mouse prion protein (PrP(Sc)) to cross the intestinal epithelium when added to the mucosal side of everted intestine. RESULTS: Fish challenged with a large amount of scrapie mouse brain homogenate by either oral or parenteral routes, showed the ability to clear the majority of infectivity load. None of the fish tissues taken at different time points after oral or parenteral inoculation was able to provoke scrapie disease after intracerebral inoculation in recipient mice. However, a few recipient mice were positive for PrP(Sc )and spongiform lesions in the brain. We also showed a specific binding of PrP(Sc )to the mucosal side of fish intestine in the absence of an active uptake of the prion protein through the intestinal wall. CONCLUSION: These results indicate that scrapie 139A, and possibly BSE, is quickly removed from fish tissues despite evidence of a prion like protein in fish and of a specific binding of PrP(Sc )to the mucosal side of fish intestine. BioMed Central 2006-06-15 /pmc/articles/PMC1513558/ /pubmed/16776828 http://dx.doi.org/10.1186/1746-6148-2-21 Text en Copyright © 2006 Ingrosso et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ingrosso, Loredana Novoa, Beatriz Valle, Andrea Z Dalla Cardone, Franco Aranguren, Raquel Sbriccoli, Marco Bevivino, Simona Iriti, Marcello Liu, Quanguo Vetrugno, Vito Lu, Mei Faoro, Franco Ciappellano, Salvatore Figueras, Antonio Pocchiari, Maurizio Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title | Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title_full | Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title_fullStr | Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title_full_unstemmed | Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title_short | Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
title_sort | scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1513558/ https://www.ncbi.nlm.nih.gov/pubmed/16776828 http://dx.doi.org/10.1186/1746-6148-2-21 |
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