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High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast
Understanding the genetic basis of evolutionary adaptation is limited by our ability to efficiently identify the genomic locations of adaptive mutations. Here we describe a method that can quickly and precisely map the genetic basis of naturally and experimentally evolved complex traits using linkag...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1514788/ https://www.ncbi.nlm.nih.gov/pubmed/16856782 http://dx.doi.org/10.1371/journal.pbio.0040256 |
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author | Segrè, Ayellet V Murray, Andrew W Leu, Jun-Yi |
author_facet | Segrè, Ayellet V Murray, Andrew W Leu, Jun-Yi |
author_sort | Segrè, Ayellet V |
collection | PubMed |
description | Understanding the genetic basis of evolutionary adaptation is limited by our ability to efficiently identify the genomic locations of adaptive mutations. Here we describe a method that can quickly and precisely map the genetic basis of naturally and experimentally evolved complex traits using linkage analysis. A yeast strain that expresses the evolved trait is crossed to a distinct strain background and DNA from a large pool of progeny that express the trait of interest is hybridized to oligonucleotide microarrays that detect thousands of polymorphisms between the two strains. Adaptive mutations are detected by linkage to the polymorphisms from the evolved parent. We successfully tested our method by mapping five known genes to a precision of 0.2–24 kb (0.1–10 cM), and developed computer simulations to test the effect of different factors on mapping precision. We then applied this method to four yeast strains that had independently adapted to a fluctuating glucose–galactose environment. All four strains had acquired one or more missense mutations in GAL80, the repressor of the galactose utilization pathway. When transferred into the ancestral strain, the gal80 mutations conferred the fitness advantage that the evolved strains show in the transition from glucose to galactose. Our results show an example of parallel adaptation caused by mutations in the same gene. |
format | Text |
id | pubmed-1514788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-15147882006-08-16 High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast Segrè, Ayellet V Murray, Andrew W Leu, Jun-Yi PLoS Biol Research Article Understanding the genetic basis of evolutionary adaptation is limited by our ability to efficiently identify the genomic locations of adaptive mutations. Here we describe a method that can quickly and precisely map the genetic basis of naturally and experimentally evolved complex traits using linkage analysis. A yeast strain that expresses the evolved trait is crossed to a distinct strain background and DNA from a large pool of progeny that express the trait of interest is hybridized to oligonucleotide microarrays that detect thousands of polymorphisms between the two strains. Adaptive mutations are detected by linkage to the polymorphisms from the evolved parent. We successfully tested our method by mapping five known genes to a precision of 0.2–24 kb (0.1–10 cM), and developed computer simulations to test the effect of different factors on mapping precision. We then applied this method to four yeast strains that had independently adapted to a fluctuating glucose–galactose environment. All four strains had acquired one or more missense mutations in GAL80, the repressor of the galactose utilization pathway. When transferred into the ancestral strain, the gal80 mutations conferred the fitness advantage that the evolved strains show in the transition from glucose to galactose. Our results show an example of parallel adaptation caused by mutations in the same gene. Public Library of Science 2006-08 2006-07-25 /pmc/articles/PMC1514788/ /pubmed/16856782 http://dx.doi.org/10.1371/journal.pbio.0040256 Text en Copyright: © 2006 Segrè et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Segrè, Ayellet V Murray, Andrew W Leu, Jun-Yi High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title | High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title_full | High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title_fullStr | High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title_full_unstemmed | High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title_short | High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast |
title_sort | high-resolution mutation mapping reveals parallel experimental evolution in yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1514788/ https://www.ncbi.nlm.nih.gov/pubmed/16856782 http://dx.doi.org/10.1371/journal.pbio.0040256 |
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