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Self-assembly of proteins and their nucleic acids

We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein...

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Detalles Bibliográficos
Autores principales: Fletcher, Graham, Mason, Sean, Terrett, Jon, Soloviev, Mikhail
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC151556/
https://www.ncbi.nlm.nih.gov/pubmed/12646068
http://dx.doi.org/10.1186/1477-3155-1-1
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author Fletcher, Graham
Mason, Sean
Terrett, Jon
Soloviev, Mikhail
author_facet Fletcher, Graham
Mason, Sean
Terrett, Jon
Soloviev, Mikhail
author_sort Fletcher, Graham
collection PubMed
description We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein and its genetic code. The principle is demonstrated using a streptavidin-based protein vector (SAPV) as both a nucleic acid binding pocket and a protein display system. We have shown that functional proteins or protein domains can be produced in vitro and physically linked to their DNA in a single enzymatic reaction. Such self-assembled protein-DNA complexes can be used for protein cloning, the cloning of protein affinity reagents or for the production of proteins which self-assemble on a variety of solid supports. Self-assembly can be utilised for making libraries of protein-DNA complexes or for labelling the protein part of such a complex to a high specific activity by labelling the nucleic acid associated with the protein. In summary, self-assembly offers an opportunity to quickly generate cheap protein affinity reagents, which can also be efficiently labelled, for use in traditional affinity assays or for protein arrays instead of conventional antibodies.
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spelling pubmed-1515562003-03-18 Self-assembly of proteins and their nucleic acids Fletcher, Graham Mason, Sean Terrett, Jon Soloviev, Mikhail J Nanobiotechnology Research We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein and its genetic code. The principle is demonstrated using a streptavidin-based protein vector (SAPV) as both a nucleic acid binding pocket and a protein display system. We have shown that functional proteins or protein domains can be produced in vitro and physically linked to their DNA in a single enzymatic reaction. Such self-assembled protein-DNA complexes can be used for protein cloning, the cloning of protein affinity reagents or for the production of proteins which self-assemble on a variety of solid supports. Self-assembly can be utilised for making libraries of protein-DNA complexes or for labelling the protein part of such a complex to a high specific activity by labelling the nucleic acid associated with the protein. In summary, self-assembly offers an opportunity to quickly generate cheap protein affinity reagents, which can also be efficiently labelled, for use in traditional affinity assays or for protein arrays instead of conventional antibodies. BioMed Central 2003-01-28 /pmc/articles/PMC151556/ /pubmed/12646068 http://dx.doi.org/10.1186/1477-3155-1-1 Text en Copyright © 2003 Fletcher et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research
Fletcher, Graham
Mason, Sean
Terrett, Jon
Soloviev, Mikhail
Self-assembly of proteins and their nucleic acids
title Self-assembly of proteins and their nucleic acids
title_full Self-assembly of proteins and their nucleic acids
title_fullStr Self-assembly of proteins and their nucleic acids
title_full_unstemmed Self-assembly of proteins and their nucleic acids
title_short Self-assembly of proteins and their nucleic acids
title_sort self-assembly of proteins and their nucleic acids
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC151556/
https://www.ncbi.nlm.nih.gov/pubmed/12646068
http://dx.doi.org/10.1186/1477-3155-1-1
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