Cargando…

Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.

Confidence in results from monitoring genetic end points in environmentally or occupationally exposed individuals can be improved with knowledge of the normal variability of changes in genetic end points in the general population. Confounding effects can be determined, and study interpretation can b...

Descripción completa

Detalles Bibliográficos
Autores principales: Anderson, D, Francis, A J, Godbert, P, Jenkinson, P C, Butterworth, K R
Formato: Texto
Lenguaje:English
Publicado: 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1521154/
https://www.ncbi.nlm.nih.gov/pubmed/8143652
_version_ 1782128779547639808
author Anderson, D
Francis, A J
Godbert, P
Jenkinson, P C
Butterworth, K R
author_facet Anderson, D
Francis, A J
Godbert, P
Jenkinson, P C
Butterworth, K R
author_sort Anderson, D
collection PubMed
description Confidence in results from monitoring genetic end points in environmentally or occupationally exposed individuals can be improved with knowledge of the normal variability of changes in genetic end points in the general population. Confounding effects can be determined, and study interpretation can be improved by correlation of this variability with various lifestyle factors such as sex and age, smoking and drinking habits, viral infections, exposure to diagnostic X-rays, etc. Eight blood samples were taken from each of 24 male and 24 female volunteers over a period of 2 years. Questionnaires pertaining to lifestyle were completed at the time of each sampling. Whole blood was cultured and slides prepared for chromosome aberration (CA) or sister chromatid exchange (SCE) analysis. Separated mononuclear cells were cultured with a range of phytohemagglutinin concentrations, and the maximum level of mitogen-induced blastogenesis was determined by measurement of [3H]thymidine uptake. There was a significant effect of both year and season of sampling for all three end points. Because there was no consistent pattern in 2 successive years, effects were thought to be independent of season. No significant effects in any of the three end points were found with respect to sex or age nor any of the other lifestyle factors, although SCE frequency and mitogen-induced blastogenesis were nearly always higher in females than in males. These results point to the need for concurrent sampling of controls with exposed populations.
format Text
id pubmed-1521154
institution National Center for Biotechnology Information
language English
publishDate 1993
record_format MEDLINE/PubMed
spelling pubmed-15211542006-07-26 Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals. Anderson, D Francis, A J Godbert, P Jenkinson, P C Butterworth, K R Environ Health Perspect Research Article Confidence in results from monitoring genetic end points in environmentally or occupationally exposed individuals can be improved with knowledge of the normal variability of changes in genetic end points in the general population. Confounding effects can be determined, and study interpretation can be improved by correlation of this variability with various lifestyle factors such as sex and age, smoking and drinking habits, viral infections, exposure to diagnostic X-rays, etc. Eight blood samples were taken from each of 24 male and 24 female volunteers over a period of 2 years. Questionnaires pertaining to lifestyle were completed at the time of each sampling. Whole blood was cultured and slides prepared for chromosome aberration (CA) or sister chromatid exchange (SCE) analysis. Separated mononuclear cells were cultured with a range of phytohemagglutinin concentrations, and the maximum level of mitogen-induced blastogenesis was determined by measurement of [3H]thymidine uptake. There was a significant effect of both year and season of sampling for all three end points. Because there was no consistent pattern in 2 successive years, effects were thought to be independent of season. No significant effects in any of the three end points were found with respect to sex or age nor any of the other lifestyle factors, although SCE frequency and mitogen-induced blastogenesis were nearly always higher in females than in males. These results point to the need for concurrent sampling of controls with exposed populations. 1993-10 /pmc/articles/PMC1521154/ /pubmed/8143652 Text en
spellingShingle Research Article
Anderson, D
Francis, A J
Godbert, P
Jenkinson, P C
Butterworth, K R
Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title_full Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title_fullStr Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title_full_unstemmed Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title_short Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
title_sort variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1521154/
https://www.ncbi.nlm.nih.gov/pubmed/8143652
work_keys_str_mv AT andersond variabilityinchromosomeaberrationssisterchromatidexchangesandmitogeninducedblastogenesisinperipherallymphocytesfromcontrolindividuals
AT francisaj variabilityinchromosomeaberrationssisterchromatidexchangesandmitogeninducedblastogenesisinperipherallymphocytesfromcontrolindividuals
AT godbertp variabilityinchromosomeaberrationssisterchromatidexchangesandmitogeninducedblastogenesisinperipherallymphocytesfromcontrolindividuals
AT jenkinsonpc variabilityinchromosomeaberrationssisterchromatidexchangesandmitogeninducedblastogenesisinperipherallymphocytesfromcontrolindividuals
AT butterworthkr variabilityinchromosomeaberrationssisterchromatidexchangesandmitogeninducedblastogenesisinperipherallymphocytesfromcontrolindividuals