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Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice

Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA-binding protein that participates in the regulation of DNA repair and maintenance of genomic integrity. In addition, PARP-1 has a role in several models of inflammation disease, where its absence or inactivation confers protection. The aim of t...

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Autores principales: García, Samuel, Bodaño, Ana, González, Antonio, Forteza, Jerónimo, Gómez-Reino, Juan J, Conde, Carmen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526570/
https://www.ncbi.nlm.nih.gov/pubmed/16356201
http://dx.doi.org/10.1186/ar1865
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author García, Samuel
Bodaño, Ana
González, Antonio
Forteza, Jerónimo
Gómez-Reino, Juan J
Conde, Carmen
author_facet García, Samuel
Bodaño, Ana
González, Antonio
Forteza, Jerónimo
Gómez-Reino, Juan J
Conde, Carmen
author_sort García, Samuel
collection PubMed
description Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA-binding protein that participates in the regulation of DNA repair and maintenance of genomic integrity. In addition, PARP-1 has a role in several models of inflammation disease, where its absence or inactivation confers protection. The aim of this study was to analyze the impact of selective PARP-1 suppression in collagen antibody-induced arthritis. We show that PARP-1 deficiency partially reduces the severity of arthritis, although the incidence of disease was similar in control and deficient mice. Decreased clinical scores were accompanied by partial reduction of histopathological findings. Interestingly, quantitative real-time PCR and ELISA analysis revealed that the absence of PARP-1 down-regulated IL-1β and monocyte chemotactic protein 1 expression in arthritic joints whereas tumor necrosis factor-α transcription was not impaired. Our results provide evidence of the contribution of PARP-1 to the progression of arthritis and identify this protein as a potential therapeutic target for the treatment of rheumatoid arthritis.
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spelling pubmed-15265702006-08-04 Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice García, Samuel Bodaño, Ana González, Antonio Forteza, Jerónimo Gómez-Reino, Juan J Conde, Carmen Arthritis Res Ther Research Article Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA-binding protein that participates in the regulation of DNA repair and maintenance of genomic integrity. In addition, PARP-1 has a role in several models of inflammation disease, where its absence or inactivation confers protection. The aim of this study was to analyze the impact of selective PARP-1 suppression in collagen antibody-induced arthritis. We show that PARP-1 deficiency partially reduces the severity of arthritis, although the incidence of disease was similar in control and deficient mice. Decreased clinical scores were accompanied by partial reduction of histopathological findings. Interestingly, quantitative real-time PCR and ELISA analysis revealed that the absence of PARP-1 down-regulated IL-1β and monocyte chemotactic protein 1 expression in arthritic joints whereas tumor necrosis factor-α transcription was not impaired. Our results provide evidence of the contribution of PARP-1 to the progression of arthritis and identify this protein as a potential therapeutic target for the treatment of rheumatoid arthritis. BioMed Central 2006 2005-12-06 /pmc/articles/PMC1526570/ /pubmed/16356201 http://dx.doi.org/10.1186/ar1865 Text en Copyright © 2005 García et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
García, Samuel
Bodaño, Ana
González, Antonio
Forteza, Jerónimo
Gómez-Reino, Juan J
Conde, Carmen
Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title_full Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title_fullStr Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title_full_unstemmed Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title_short Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
title_sort partial protection against collagen antibody-induced arthritis in parp-1 deficient mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526570/
https://www.ncbi.nlm.nih.gov/pubmed/16356201
http://dx.doi.org/10.1186/ar1865
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