Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summ...

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Detalles Bibliográficos
Autores principales: Yang, Xiaohan, Scheffler, Brian E, Weston, Leslie A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526718/
https://www.ncbi.nlm.nih.gov/pubmed/16509990
http://dx.doi.org/10.1186/1746-4811-2-4
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author Yang, Xiaohan
Scheffler, Brian E
Weston, Leslie A
author_facet Yang, Xiaohan
Scheffler, Brian E
Weston, Leslie A
author_sort Yang, Xiaohan
collection PubMed
description Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.
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spelling pubmed-15267182006-08-04 Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants Yang, Xiaohan Scheffler, Brian E Weston, Leslie A Plant Methods Review Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants. BioMed Central 2006-03-01 /pmc/articles/PMC1526718/ /pubmed/16509990 http://dx.doi.org/10.1186/1746-4811-2-4 Text en Copyright © 2006 Yang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Yang, Xiaohan
Scheffler, Brian E
Weston, Leslie A
Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title_full Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title_fullStr Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title_full_unstemmed Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title_short Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
title_sort recent developments in primer design for dna polymorphism and mrna profiling in higher plants
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526718/
https://www.ncbi.nlm.nih.gov/pubmed/16509990
http://dx.doi.org/10.1186/1746-4811-2-4
work_keys_str_mv AT yangxiaohan recentdevelopmentsinprimerdesignfordnapolymorphismandmrnaprofilinginhigherplants
AT schefflerbriane recentdevelopmentsinprimerdesignfordnapolymorphismandmrnaprofilinginhigherplants
AT westonlesliea recentdevelopmentsinprimerdesignfordnapolymorphismandmrnaprofilinginhigherplants

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