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Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats

Proinflammatory (TNF-α, IL-1β, and NO) and antiinflammatory (IL-10, CO) levels were assayed in serum, liver, and small bowel in order to verify a hypothetic inflammatory etiopathogeny of portal hypertension that could be the cause of its evolutive heterogeneity. Male Wistar rats were divided into on...

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Autores principales: Aller, Maria Angeles, Vara, Elena, Garcia, Cruz, Palma, Maria Dolores, Arias, Jorge L., Nava, Maria Paz, Arias, Jaime
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533904/
https://www.ncbi.nlm.nih.gov/pubmed/16030393
http://dx.doi.org/10.1155/MI.2005.101
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author Aller, Maria Angeles
Vara, Elena
Garcia, Cruz
Palma, Maria Dolores
Arias, Jorge L.
Nava, Maria Paz
Arias, Jaime
author_facet Aller, Maria Angeles
Vara, Elena
Garcia, Cruz
Palma, Maria Dolores
Arias, Jorge L.
Nava, Maria Paz
Arias, Jaime
author_sort Aller, Maria Angeles
collection PubMed
description Proinflammatory (TNF-α, IL-1β, and NO) and antiinflammatory (IL-10, CO) levels were assayed in serum, liver, and small bowel in order to verify a hypothetic inflammatory etiopathogeny of portal hypertension that could be the cause of its evolutive heterogeneity. Male Wistar rats were divided into one control group (n = 11) and one group with a triple stenosing ligation of the portal vein (n = 23) after 28 days of evolution. In one subgroup of portal hypertensive rats, portal pressure, collateral venous circulation, mesenteric vasculopathy, and liver and spleen weights were determined. In the remaining rats with portal hypertension TNF-α, IL-1β, and IL-10 were quantified in liver and ileum by enzyme-linked immunosorbent assay. NO synthase activity was studied in liver and ileum. CO and NO were measured in portal and systemic blood by spectrophotometry and Griess reaction, respectively. Portal hypertensive rats with mayor spleen weight show hepatomegaly and mayor development of collateral circulation. Ileum release of IL-10 (0.30 ± 0.12 versus 0.14 ± 0.02 pmol/mg protein; P < .01) is associated with a liver production of both proinflammatory mediators (TNF-α: 2 ± 0.21 versus 1.32 ± 0.60 pmol/mg protein; P < .05, IL-1β: 19.17 ± 2.87 versus 5.96 ± 1.84 pmol/mg protein; P = .005, and NO: 132.10 ± 34.72 versus 61.05 ± 8.30 nmol/mL; P = .005) and an antiinflammatory mediator (CO: 6.49 ± 2.99 versus 3.03 ± 1.59 pmol/mL; P = .005). In short-term prehepatic portal hypertension a gut-liver inflammatory loop, which could be fundamental in the regulation both of the portal pressure and of its complications, could be proposed.
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spelling pubmed-15339042006-08-21 Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats Aller, Maria Angeles Vara, Elena Garcia, Cruz Palma, Maria Dolores Arias, Jorge L. Nava, Maria Paz Arias, Jaime Mediators Inflamm Research Communication Proinflammatory (TNF-α, IL-1β, and NO) and antiinflammatory (IL-10, CO) levels were assayed in serum, liver, and small bowel in order to verify a hypothetic inflammatory etiopathogeny of portal hypertension that could be the cause of its evolutive heterogeneity. Male Wistar rats were divided into one control group (n = 11) and one group with a triple stenosing ligation of the portal vein (n = 23) after 28 days of evolution. In one subgroup of portal hypertensive rats, portal pressure, collateral venous circulation, mesenteric vasculopathy, and liver and spleen weights were determined. In the remaining rats with portal hypertension TNF-α, IL-1β, and IL-10 were quantified in liver and ileum by enzyme-linked immunosorbent assay. NO synthase activity was studied in liver and ileum. CO and NO were measured in portal and systemic blood by spectrophotometry and Griess reaction, respectively. Portal hypertensive rats with mayor spleen weight show hepatomegaly and mayor development of collateral circulation. Ileum release of IL-10 (0.30 ± 0.12 versus 0.14 ± 0.02 pmol/mg protein; P < .01) is associated with a liver production of both proinflammatory mediators (TNF-α: 2 ± 0.21 versus 1.32 ± 0.60 pmol/mg protein; P < .05, IL-1β: 19.17 ± 2.87 versus 5.96 ± 1.84 pmol/mg protein; P = .005, and NO: 132.10 ± 34.72 versus 61.05 ± 8.30 nmol/mL; P = .005) and an antiinflammatory mediator (CO: 6.49 ± 2.99 versus 3.03 ± 1.59 pmol/mL; P = .005). In short-term prehepatic portal hypertension a gut-liver inflammatory loop, which could be fundamental in the regulation both of the portal pressure and of its complications, could be proposed. Hindawi Publishing Corporation 2005-06-09 /pmc/articles/PMC1533904/ /pubmed/16030393 http://dx.doi.org/10.1155/MI.2005.101 Text en Hindawi Publishing Corporation
spellingShingle Research Communication
Aller, Maria Angeles
Vara, Elena
Garcia, Cruz
Palma, Maria Dolores
Arias, Jorge L.
Nava, Maria Paz
Arias, Jaime
Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title_full Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title_fullStr Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title_full_unstemmed Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title_short Proinflammatory Liver and Antiinflammatory Intestinal Mediators Involved in Portal Hypertensive Rats
title_sort proinflammatory liver and antiinflammatory intestinal mediators involved in portal hypertensive rats
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533904/
https://www.ncbi.nlm.nih.gov/pubmed/16030393
http://dx.doi.org/10.1155/MI.2005.101
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