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Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance
BACKGROUND: A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20–200 micro-grams total RNA or 0.5–2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from large,...
| Autores principales: | , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC153514/ https://www.ncbi.nlm.nih.gov/pubmed/12659661 http://dx.doi.org/10.1186/1471-2164-4-11 |