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Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway

BACKGROUND: Floral scent is one of the important strategies for ensuring fertilization and for determining seed or fruit set. Research on plant scents has hampered mainly by the invisibility of this character, its dynamic nature, and complex mixtures of components that are present in very small quan...

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Autores principales: Hsiao, Yu-Yun, Tsai, Wen-Chieh, Kuoh, Chang-Sheng, Huang, Tian-Hsiang, Wang, Hei-Chia, Wu, Tian-Shung, Leu, Yann-Lii, Chen, Wen-Huei, Chen, Hong-Hwa
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540424/
https://www.ncbi.nlm.nih.gov/pubmed/16836766
http://dx.doi.org/10.1186/1471-2229-6-14
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author Hsiao, Yu-Yun
Tsai, Wen-Chieh
Kuoh, Chang-Sheng
Huang, Tian-Hsiang
Wang, Hei-Chia
Wu, Tian-Shung
Leu, Yann-Lii
Chen, Wen-Huei
Chen, Hong-Hwa
author_facet Hsiao, Yu-Yun
Tsai, Wen-Chieh
Kuoh, Chang-Sheng
Huang, Tian-Hsiang
Wang, Hei-Chia
Wu, Tian-Shung
Leu, Yann-Lii
Chen, Wen-Huei
Chen, Hong-Hwa
author_sort Hsiao, Yu-Yun
collection PubMed
description BACKGROUND: Floral scent is one of the important strategies for ensuring fertilization and for determining seed or fruit set. Research on plant scents has hampered mainly by the invisibility of this character, its dynamic nature, and complex mixtures of components that are present in very small quantities. Most progress in scent research, as in other areas of plant biology, has come from the use of molecular and biochemical techniques. Although volatile components have been identified in several orchid species, the biosynthetic pathways of orchid flower fragrance are far from understood. We investigated how flower fragrance was generated in certain Phalaenopsis orchids by determining the chemical components of the floral scent, identifying floral expressed-sequence-tags (ESTs), and deducing the pathways of floral scent biosynthesis in Phalaneopsis bellina by bioinformatics analysis. RESULTS: The main chemical components in the P. bellina flower were shown by gas chromatography-mass spectrometry to be monoterpenoids, benzenoids and phenylpropanoids. The set of floral scent producing enzymes in the biosynthetic pathway from glyceraldehyde-3-phosphate (G3P) to geraniol and linalool were recognized through data mining of the P. bellina floral EST database (dbEST). Transcripts preferentially expressed in P. bellina were distinguished by comparing the scent floral dbEST to that of a scentless species, P. equestris, and included those encoding lipoxygenase, epimerase, diacylglycerol kinase and geranyl diphosphate synthase. In addition, EST filtering results showed that transcripts encoding signal transduction and Myb transcription factors and methyltransferase, in addition to those for scent biosynthesis, were detected by in silico hybridization of the P. bellina unigene database against those of the scentless species, rice and Arabidopsis. Altogether, we pinpointed 66% of the biosynthetic steps from G3P to geraniol, linalool and their derivatives. CONCLUSION: This systems biology program combined chemical analysis, genomics and bioinformatics to elucidate the scent biosynthesis pathway and identify the relevant genes. It integrates the forward and reverse genetic approaches to knowledge discovery by which researchers can study non-model plants.
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spelling pubmed-15404242006-08-12 Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway Hsiao, Yu-Yun Tsai, Wen-Chieh Kuoh, Chang-Sheng Huang, Tian-Hsiang Wang, Hei-Chia Wu, Tian-Shung Leu, Yann-Lii Chen, Wen-Huei Chen, Hong-Hwa BMC Plant Biol Research Article BACKGROUND: Floral scent is one of the important strategies for ensuring fertilization and for determining seed or fruit set. Research on plant scents has hampered mainly by the invisibility of this character, its dynamic nature, and complex mixtures of components that are present in very small quantities. Most progress in scent research, as in other areas of plant biology, has come from the use of molecular and biochemical techniques. Although volatile components have been identified in several orchid species, the biosynthetic pathways of orchid flower fragrance are far from understood. We investigated how flower fragrance was generated in certain Phalaenopsis orchids by determining the chemical components of the floral scent, identifying floral expressed-sequence-tags (ESTs), and deducing the pathways of floral scent biosynthesis in Phalaneopsis bellina by bioinformatics analysis. RESULTS: The main chemical components in the P. bellina flower were shown by gas chromatography-mass spectrometry to be monoterpenoids, benzenoids and phenylpropanoids. The set of floral scent producing enzymes in the biosynthetic pathway from glyceraldehyde-3-phosphate (G3P) to geraniol and linalool were recognized through data mining of the P. bellina floral EST database (dbEST). Transcripts preferentially expressed in P. bellina were distinguished by comparing the scent floral dbEST to that of a scentless species, P. equestris, and included those encoding lipoxygenase, epimerase, diacylglycerol kinase and geranyl diphosphate synthase. In addition, EST filtering results showed that transcripts encoding signal transduction and Myb transcription factors and methyltransferase, in addition to those for scent biosynthesis, were detected by in silico hybridization of the P. bellina unigene database against those of the scentless species, rice and Arabidopsis. Altogether, we pinpointed 66% of the biosynthetic steps from G3P to geraniol, linalool and their derivatives. CONCLUSION: This systems biology program combined chemical analysis, genomics and bioinformatics to elucidate the scent biosynthesis pathway and identify the relevant genes. It integrates the forward and reverse genetic approaches to knowledge discovery by which researchers can study non-model plants. BioMed Central 2006-07-13 /pmc/articles/PMC1540424/ /pubmed/16836766 http://dx.doi.org/10.1186/1471-2229-6-14 Text en Copyright © 2006 Hsiao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hsiao, Yu-Yun
Tsai, Wen-Chieh
Kuoh, Chang-Sheng
Huang, Tian-Hsiang
Wang, Hei-Chia
Wu, Tian-Shung
Leu, Yann-Lii
Chen, Wen-Huei
Chen, Hong-Hwa
Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title_full Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title_fullStr Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title_full_unstemmed Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title_short Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway
title_sort comparison of transcripts in phalaenopsis bellina and phalaenopsis equestris (orchidaceae) flowers to deduce monoterpene biosynthesis pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540424/
https://www.ncbi.nlm.nih.gov/pubmed/16836766
http://dx.doi.org/10.1186/1471-2229-6-14
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