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Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue

BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid...

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Detalles Bibliográficos
Autores principales: Smith, Robert A, Lea, Rod A, Curran, Joanne E, Weinstein, Stephen R, Griffiths, Lyn R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC154135/
https://www.ncbi.nlm.nih.gov/pubmed/12559052
http://dx.doi.org/10.1186/bcr556
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author Smith, Robert A
Lea, Rod A
Curran, Joanne E
Weinstein, Stephen R
Griffiths, Lyn R
author_facet Smith, Robert A
Lea, Rod A
Curran, Joanne E
Weinstein, Stephen R
Griffiths, Lyn R
author_sort Smith, Robert A
collection PubMed
description BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. METHODS: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. RESULTS: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). CONCLUSION: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.
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spelling pubmed-1541352003-05-06 Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue Smith, Robert A Lea, Rod A Curran, Joanne E Weinstein, Stephen R Griffiths, Lyn R Breast Cancer Res Research Article BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. METHODS: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. RESULTS: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). CONCLUSION: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue. BioMed Central 2003 2002-11-07 /pmc/articles/PMC154135/ /pubmed/12559052 http://dx.doi.org/10.1186/bcr556 Text en Copyright © 2003 Smith et al., licensee BioMed Central Ltd
spellingShingle Research Article
Smith, Robert A
Lea, Rod A
Curran, Joanne E
Weinstein, Stephen R
Griffiths, Lyn R
Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title_full Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title_fullStr Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title_full_unstemmed Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title_short Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
title_sort expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC154135/
https://www.ncbi.nlm.nih.gov/pubmed/12559052
http://dx.doi.org/10.1186/bcr556
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