Cargando…
Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis
BACKGROUND: The Helicase-Like Transcription Factor (HLTF/SMARCA3) belongs to the family of SWI/SNF proteins that use the energy of ATP hydrolysis to remodel chromatin in a variety of cellular processes. Several SWI/SNF genes are disrupted in cancer, suggesting a role of tumor suppressor. Similarly,...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1550248/ https://www.ncbi.nlm.nih.gov/pubmed/16762066 http://dx.doi.org/10.1186/1476-4598-5-23 |
_version_ | 1782129211810512896 |
---|---|
author | Debauve, Gaël Nonclercq, Denis Ribaucour, Fabrice Wiedig, Murielle Gerbaux, Cécile Leo, Oberdan Laurent, Guy Journé, Fabrice Belayew, Alexandra Toubeau, Gérard |
author_facet | Debauve, Gaël Nonclercq, Denis Ribaucour, Fabrice Wiedig, Murielle Gerbaux, Cécile Leo, Oberdan Laurent, Guy Journé, Fabrice Belayew, Alexandra Toubeau, Gérard |
author_sort | Debauve, Gaël |
collection | PubMed |
description | BACKGROUND: The Helicase-Like Transcription Factor (HLTF/SMARCA3) belongs to the family of SWI/SNF proteins that use the energy of ATP hydrolysis to remodel chromatin in a variety of cellular processes. Several SWI/SNF genes are disrupted in cancer, suggesting a role of tumor suppressor. Similarly, the HLTF gene was recently found to be inactivated by hypermethylation in a number of advanced colon and gastric tumors. However, other evidences indicated a 20-fold HLTF overexpression in cell lines derived from various neoplasms (ovary, breast, cervix, kidney...). RESULTS: In the present study, we investigated HLTF expression by immunohistochemistry in a model of kidney tumors induced by continuous administration of diethylstilbestrol to male Syrian golden hamsters. A strong labeling was already detected in small tumor buds, making HLTF an early cancer marker in this model. Although every cell stained for HLTF at this early stage, the number of HLTF-positive cells decreased to 10% with cancer progression, and these positive cells were dispersed in the tumor mass. HLTF expression was conserved in the HKT-1097 cell line established from kidney tumors, but again only 10% of positive cells were found in xenografts produced by HKT-1097 cells in nude mice. CONCLUSION: In conclusion, our data suggest that HLTF gene activation is linked to initial steps of carcinogenesis in this model and should be investigated in early stages of other neoplasms. |
format | Text |
id | pubmed-1550248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15502482006-08-17 Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis Debauve, Gaël Nonclercq, Denis Ribaucour, Fabrice Wiedig, Murielle Gerbaux, Cécile Leo, Oberdan Laurent, Guy Journé, Fabrice Belayew, Alexandra Toubeau, Gérard Mol Cancer Research BACKGROUND: The Helicase-Like Transcription Factor (HLTF/SMARCA3) belongs to the family of SWI/SNF proteins that use the energy of ATP hydrolysis to remodel chromatin in a variety of cellular processes. Several SWI/SNF genes are disrupted in cancer, suggesting a role of tumor suppressor. Similarly, the HLTF gene was recently found to be inactivated by hypermethylation in a number of advanced colon and gastric tumors. However, other evidences indicated a 20-fold HLTF overexpression in cell lines derived from various neoplasms (ovary, breast, cervix, kidney...). RESULTS: In the present study, we investigated HLTF expression by immunohistochemistry in a model of kidney tumors induced by continuous administration of diethylstilbestrol to male Syrian golden hamsters. A strong labeling was already detected in small tumor buds, making HLTF an early cancer marker in this model. Although every cell stained for HLTF at this early stage, the number of HLTF-positive cells decreased to 10% with cancer progression, and these positive cells were dispersed in the tumor mass. HLTF expression was conserved in the HKT-1097 cell line established from kidney tumors, but again only 10% of positive cells were found in xenografts produced by HKT-1097 cells in nude mice. CONCLUSION: In conclusion, our data suggest that HLTF gene activation is linked to initial steps of carcinogenesis in this model and should be investigated in early stages of other neoplasms. BioMed Central 2006-06-08 /pmc/articles/PMC1550248/ /pubmed/16762066 http://dx.doi.org/10.1186/1476-4598-5-23 Text en Copyright © 2006 Debauve et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Debauve, Gaël Nonclercq, Denis Ribaucour, Fabrice Wiedig, Murielle Gerbaux, Cécile Leo, Oberdan Laurent, Guy Journé, Fabrice Belayew, Alexandra Toubeau, Gérard Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title | Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title_full | Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title_fullStr | Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title_full_unstemmed | Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title_short | Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis |
title_sort | early expression of the helicase-like transcription factor (hltf/smarca3) in an experimental model of estrogen-induced renal carcinogenesis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1550248/ https://www.ncbi.nlm.nih.gov/pubmed/16762066 http://dx.doi.org/10.1186/1476-4598-5-23 |
work_keys_str_mv | AT debauvegael earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT nonclercqdenis earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT ribaucourfabrice earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT wiedigmurielle earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT gerbauxcecile earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT leooberdan earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT laurentguy earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT journefabrice earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT belayewalexandra earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis AT toubeaugerard earlyexpressionofthehelicaseliketranscriptionfactorhltfsmarca3inanexperimentalmodelofestrogeninducedrenalcarcinogenesis |