Cargando…

Modulation of the transcription regulatory program in yeast cells committed to sporulation

BACKGROUND: Meiosis in budding yeast is coupled to the process of sporulation, where the four haploid nuclei are packaged into a gamete. This differentiation process is characterized by a point of transition, termed commitment, when it becomes independent of the environment. Not much is known about...

Descripción completa

Detalles Bibliográficos
Autores principales: Friedlander, Gilgi, Joseph-Strauss, Daphna, Carmi, Miri, Zenvirth, Drora, Simchen, Giora, Barkai, Naama
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557749/
https://www.ncbi.nlm.nih.gov/pubmed/16542486
http://dx.doi.org/10.1186/gb-2006-7-3-r20
_version_ 1782129403702018048
author Friedlander, Gilgi
Joseph-Strauss, Daphna
Carmi, Miri
Zenvirth, Drora
Simchen, Giora
Barkai, Naama
author_facet Friedlander, Gilgi
Joseph-Strauss, Daphna
Carmi, Miri
Zenvirth, Drora
Simchen, Giora
Barkai, Naama
author_sort Friedlander, Gilgi
collection PubMed
description BACKGROUND: Meiosis in budding yeast is coupled to the process of sporulation, where the four haploid nuclei are packaged into a gamete. This differentiation process is characterized by a point of transition, termed commitment, when it becomes independent of the environment. Not much is known about the mechanisms underlying commitment, but it is often assumed that positive feedback loops stabilize the underlying gene-expression cascade. RESULTS: We describe the gene-expression program of committed cells. Sporulating cells were transferred back to growth medium at different stages of the process, and their transcription response was characterized. Most sporulation-induced genes were immediately downregulated upon transfer, even in committed cells that continued to sporulate. Focusing on the metabolic-related transcription response, we observed that pre-committed cells, as well as mature spores, responded to the transfer to growth medium in essentially the same way that vegetative cells responded to glucose. In contrast, committed cells elicited a dramatically different response. CONCLUSION: Our results suggest that cells ensure commitment to sporulation not by stabilizing the process, but by modulating their gene-expression program in an active manner. This unique transcriptional program may optimize sporulation in an environment-specific manner.
format Text
id pubmed-1557749
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-15577492006-09-01 Modulation of the transcription regulatory program in yeast cells committed to sporulation Friedlander, Gilgi Joseph-Strauss, Daphna Carmi, Miri Zenvirth, Drora Simchen, Giora Barkai, Naama Genome Biol Research BACKGROUND: Meiosis in budding yeast is coupled to the process of sporulation, where the four haploid nuclei are packaged into a gamete. This differentiation process is characterized by a point of transition, termed commitment, when it becomes independent of the environment. Not much is known about the mechanisms underlying commitment, but it is often assumed that positive feedback loops stabilize the underlying gene-expression cascade. RESULTS: We describe the gene-expression program of committed cells. Sporulating cells were transferred back to growth medium at different stages of the process, and their transcription response was characterized. Most sporulation-induced genes were immediately downregulated upon transfer, even in committed cells that continued to sporulate. Focusing on the metabolic-related transcription response, we observed that pre-committed cells, as well as mature spores, responded to the transfer to growth medium in essentially the same way that vegetative cells responded to glucose. In contrast, committed cells elicited a dramatically different response. CONCLUSION: Our results suggest that cells ensure commitment to sporulation not by stabilizing the process, but by modulating their gene-expression program in an active manner. This unique transcriptional program may optimize sporulation in an environment-specific manner. BioMed Central 2006 2006-03-08 /pmc/articles/PMC1557749/ /pubmed/16542486 http://dx.doi.org/10.1186/gb-2006-7-3-r20 Text en Copyright © 2006 Friedlander et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Friedlander, Gilgi
Joseph-Strauss, Daphna
Carmi, Miri
Zenvirth, Drora
Simchen, Giora
Barkai, Naama
Modulation of the transcription regulatory program in yeast cells committed to sporulation
title Modulation of the transcription regulatory program in yeast cells committed to sporulation
title_full Modulation of the transcription regulatory program in yeast cells committed to sporulation
title_fullStr Modulation of the transcription regulatory program in yeast cells committed to sporulation
title_full_unstemmed Modulation of the transcription regulatory program in yeast cells committed to sporulation
title_short Modulation of the transcription regulatory program in yeast cells committed to sporulation
title_sort modulation of the transcription regulatory program in yeast cells committed to sporulation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557749/
https://www.ncbi.nlm.nih.gov/pubmed/16542486
http://dx.doi.org/10.1186/gb-2006-7-3-r20
work_keys_str_mv AT friedlandergilgi modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation
AT josephstraussdaphna modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation
AT carmimiri modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation
AT zenvirthdrora modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation
AT simchengiora modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation
AT barkainaama modulationofthetranscriptionregulatoryprograminyeastcellscommittedtosporulation